Hobi-Like Pestivirus in Aborted Bovine Fetuses

被引：60

作者：

Decaro, Nicola ^{[1
]}

Lucente, Maria Stella ^{[1
]}

Mari, Viviana ^{[1
]}

Sciarretta, Rossana ^{[1
]}

Pinto, Pierfrancesco ^{[1
]}

Buonavoglia, Domenico ^{[1
]}

Martella, Vito ^{[1
]}

Buonavoglia, Canio ^{[1
]}

机构：

[1] Fac Vet Med, Dept Vet Publ Hlth, Bari, Italy

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2012年 / 50卷 / 02期

关键词：

REAL-TIME PCR; ATYPICAL PESTIVIRUS; RT-PCR; ASSAY;

D O I：

10.1128/JCM.05887-11

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

An outbreak of abortion affecting multiparous cows was associated with Hobi-like pestivirus infection. Viral RNA and antigens were detected in the tissues of two aborted fetuses. Molecular assays for other common abortogenic agents tested negative. At the genetic level, the Hobi-like pestivirus displayed the closest relatedness to Italian, Australian, and South American viruses, whereas it diverged from the prototype Thai isolate. These findings may have important implications for the pestivirus control/eradication programs in cattle herds.

引用

收藏

页码：509 / 512

页数：4

相关论文

共 27 条

[1] Evaluation of genus-specific and species-specific real-time PCR assays for the identification of Brucella spp. [J].

Al Dahouk, Sascha ;

Noeckler, Karsten ;

Scholz, Holger C. ;

Pfeffer, Martin ;

Neubauer, Heinrich ;

Tomaso, Herbert .

CLINICAL CHEMISTRY AND LABORATORY MEDICINE, 2007, 45 (11) :1464-1470

[2] THE CLINICAL MANIFESTATIONS OF BOVINE VIRAL DIARRHEA INFECTION [J].

BAKER, JC .

VETERINARY CLINICS OF NORTH AMERICA-FOOD ANIMAL PRACTICE, 1995, 11 (03) :425-&

[3] Bluetongue virus diagnosis of clinical cases by a duplex reverse transcription-PCR: a comparison with conventional methods [J].

Billinis, C ;

Koumbati, M ;

Spyrou, V ;

Nomikou, K ;

Mangana, O ;

Panagiotidis, CA ;

Papadopoulos, O .

JOURNAL OF VIROLOGICAL METHODS, 2001, 98 (01) :77-89

[4] Genetic characterization of Brazilian bovine viral diarrhea virus isolates by partial nucleotide sequencing of the 5′-UTR region [J].

Cortez, Adriana ;

Heinemann, Marcos B. ;

de Castro, Alessandra Marnie M. G. ;

Soares, Rodrigo M. ;

Pinto, Ana Maria V. ;

Alfieri, Amauri A. ;

Flores, Eduardo F. ;

Leite, Romulo Cerqueira ;

Richtzenhain, Leonardo J. .

PESQUISA VETERINARIA BRASILEIRA, 2006, 26 (04) :211-216

[5] Experimental infection of cattle, sheep and pigs with 'Hobi'-like pestivirus [J].

Decaro, Nicola ;

Mari, Viviana ;

Lucente, Maria Stella ;

Sciarretta, Rossana ;

Moreno, Ana ;

Armenise, Carlo ;

Losurdo, Michele ;

Camero, Michele ;

Lorusso, Eleonora ;

Cordioli, Paolo ;

Buonavoglia, Canio .

VETERINARY MICROBIOLOGY, 2012, 155 (2-4) :165-171

[6] A nested PCR approach for unambiguous typing of pestiviruses infecting cattle [J].

Decaro, Nicola ;

Sciarretta, Rossana ;

Lucente, Maria Stella ;

Mari, Viviana ;

Amorisco, Francesca ;

Colaianni, Maria Loredana ;

Cordioli, Paolo ;

Parisi, Antonio ;

Lelli, Rossella ;

Buonavoglia, Canio .

MOLECULAR AND CELLULAR PROBES, 2012, 26 (01) :42-46

[7] Atypical Pestivirus and Severe Respiratory Disease in Calves, Europe [J].

Decaro, Nicola ;

Lucente, Maria Stella ;

Mari, Viviana ;

Cirone, Francesco ;

Cordioli, Paolo ;

Camero, Michele ;

Sciarretta, Rossana ;

Losurdo, Michele ;

Lorusso, Eleonora ;

Buonavoglia, Canio .

EMERGING INFECTIOUS DISEASES, 2011, 17 (08) :1549-1552

[8] Use of real-time RT-PCR as a rapid molecular approach for differentiation of field and vaccine strains of bluetongue virus serotypes 2 and 9 [J].

Elia, Gabriella ;

Savini, Giovanni ;

Decaro, Nicola ;

Martella, Vito ;

Teodori, Liana ;

Casaccia, Claudia ;

Di Gialleonardo, Luigina ;

Lorusso, Eleonora ;

Caporale, Vincenzo ;

Buonavoglia, Canio .

MOLECULAR AND CELLULAR PROBES, 2008, 22 (01) :38-46

[9] Rapid detection of the Chlamydiaceae and other families in the order Chlamydiales:: Three PCR tests [J].

Everett, KDE ;

Hornung, LJ ;

Andersen, AA .

JOURNAL OF CLINICAL MICROBIOLOGY, 1999, 37 (03) :575-580

[10]

Hall TA., 1999, NUCL ACIDS S SERIES, V41, P95, DOI DOI 10.14344/IOC.ML.11.1