Available Toolkits for CRISPR/CAS Genome Editing in Plants

被引：4

作者：

Mikhaylova, E., V ^{[1
]}

Khusnutdinov, E. A. ^{[1
]}

Chemeris, A., V ^{[1
]}

Kuluev, B. R. ^{[1
]}

机构：

[1] Russian Acad Sci, Ufa Fed Res Ctr, Inst Biochem & Genet, Ufa, Russia

来源：

RUSSIAN JOURNAL OF PLANT PHYSIOLOGY | 2022年 / 69卷 / 01期

基金：

俄罗斯科学基金会;

关键词：

genome editing; CRISPR; Cas9; Cas12a; Cas12b; Csy4; Ec1; 2; geminiviruses; knockout; knock-in; TRANSCRIPTIONAL ACTIVATION; TARGETED MUTAGENESIS; SYSTEM; EFFICIENCY; PECULIARITIES; REPRESSION; VARIANTS; IMPROVE; GENES; RICE;

D O I：

10.1134/S1021443722010137

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

At present, CRISPR/Cas genome editing is the most effective and fast method to produce plants with desired traits. Unlike traditional genetic engineering, this method induces precise modifications in target sites of the genome. Therefore, in addition to applied tasks, genome editing is often used for basic investigation of native plant genes. However, the CRISPR/Cas technique has so far been tested in bacterial and animal objects rather than plants. Nevertheless, there are easy-to-use vector systems containing a wide range of elements designed for plant genome editing and regulation of target genes' expression. Researchers should only choose an appropriate system and adapt it to their object and the aim of the experiment. In this paper, opportunities offered by currently available systems for plant genome editing are reviewed in detail.

引用

页数：14

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