At present, CRISPR/Cas genome editing is the most effective and fast method to produce plants with desired traits. Unlike traditional genetic engineering, this method induces precise modifications in target sites of the genome. Therefore, in addition to applied tasks, genome editing is often used for basic investigation of native plant genes. However, the CRISPR/Cas technique has so far been tested in bacterial and animal objects rather than plants. Nevertheless, there are easy-to-use vector systems containing a wide range of elements designed for plant genome editing and regulation of target genes' expression. Researchers should only choose an appropriate system and adapt it to their object and the aim of the experiment. In this paper, opportunities offered by currently available systems for plant genome editing are reviewed in detail.
机构:
Reliance Ind Ltd, R&D Synthet Biol, Navi Mumbai, IndiaReliance Ind Ltd, R&D Synthet Biol, Navi Mumbai, India
Bandyopadhyay, Anindya
;
Kancharla, Nagesh
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Reliance Ind Ltd, R&D Synthet Biol, Navi Mumbai, IndiaReliance Ind Ltd, R&D Synthet Biol, Navi Mumbai, India
Kancharla, Nagesh
;
Javalkote, Vivek S.
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Reliance Ind Ltd, R&D Synthet Biol, Navi Mumbai, IndiaReliance Ind Ltd, R&D Synthet Biol, Navi Mumbai, India
Javalkote, Vivek S.
;
Dasgupta, Santanu
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Reliance Ind Ltd, R&D Synthet Biol, Navi Mumbai, IndiaReliance Ind Ltd, R&D Synthet Biol, Navi Mumbai, India
Dasgupta, Santanu
;
Brutnell, Thomas P.
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机构:
Chinese Acad Agr Sci, Biotechnol Res Inst, Beijing, Peoples R China
Gateway Biotechnol Inc, St Louis, MO USAReliance Ind Ltd, R&D Synthet Biol, Navi Mumbai, India
机构:
Rockefeller Univ, Bacteriol Lab, New York, NY 10065 USARockefeller Univ, Bacteriol Lab, New York, NY 10065 USA
Bikard, David
;
Jiang, Wenyan
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h-index: 0
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Rockefeller Univ, Bacteriol Lab, New York, NY 10065 USARockefeller Univ, Bacteriol Lab, New York, NY 10065 USA
Jiang, Wenyan
;
Samai, Poulami
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Rockefeller Univ, Bacteriol Lab, New York, NY 10065 USARockefeller Univ, Bacteriol Lab, New York, NY 10065 USA
Samai, Poulami
;
Hochschild, Ann
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机构:
Harvard Univ, Sch Med, Dept Microbiol & Immunobiol, Boston, MA 02115 USARockefeller Univ, Bacteriol Lab, New York, NY 10065 USA
Hochschild, Ann
;
Zhang, Feng
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机构:
Broad Inst MIT & Harvard, Cambridge, MA 02142 USA
MIT, McGovern Inst Brain Res, Cambridge, MA 02139 USA
MIT, Dept Brain & Cognit Sci, Cambridge, MA 02139 USA
MIT, Dept Biol Engn, Cambridge, MA 02139 USARockefeller Univ, Bacteriol Lab, New York, NY 10065 USA
Zhang, Feng
;
Marraffini, Luciano A.
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Rockefeller Univ, Bacteriol Lab, New York, NY 10065 USARockefeller Univ, Bacteriol Lab, New York, NY 10065 USA
机构:
USDA ARS, Vegetable Crops Res Unit, Madison, WI 53726 USA
Univ Wisconsin, Dept Hort, 1575 Linden Dr, Madison, WI 53706 USAUSDA ARS, Vegetable Crops Res Unit, Madison, WI 53726 USA
Butler, Nathaniel M.
;
Jansky, Shelley H.
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USDA ARS, Vegetable Crops Res Unit, Madison, WI 53726 USA
Univ Wisconsin, Dept Hort, 1575 Linden Dr, Madison, WI 53706 USAUSDA ARS, Vegetable Crops Res Unit, Madison, WI 53726 USA
Jansky, Shelley H.
;
Jiang, Jiming
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Michigan State Univ, Dept Hort, Dept Plant Biol, E Lansing, MI 48824 USAUSDA ARS, Vegetable Crops Res Unit, Madison, WI 53726 USA
机构:
Michigan State Univ, Dept Plant Soils & Microbial Sci, E Lansing, MI 48824 USAMichigan State Univ, Dept Plant Soils & Microbial Sci, E Lansing, MI 48824 USA
Butler, Nathaniel M.
;
Baltes, Nicholas J.
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机构:
Univ Minnesota, Dept Genet Cell Biol & Dev, Minneapolis, MN USA
Univ Minnesota, Ctr Genome Engn, Minneapolis, MN USAMichigan State Univ, Dept Plant Soils & Microbial Sci, E Lansing, MI 48824 USA
Baltes, Nicholas J.
;
Voytas, Daniel F.
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Univ Minnesota, Dept Genet Cell Biol & Dev, Minneapolis, MN USA
Univ Minnesota, Ctr Genome Engn, Minneapolis, MN USAMichigan State Univ, Dept Plant Soils & Microbial Sci, E Lansing, MI 48824 USA
Voytas, Daniel F.
;
Douches, David S.
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Michigan State Univ, Dept Plant Soils & Microbial Sci, E Lansing, MI 48824 USAMichigan State Univ, Dept Plant Soils & Microbial Sci, E Lansing, MI 48824 USA
机构:
Reliance Ind Ltd, R&D Synthet Biol, Navi Mumbai, IndiaReliance Ind Ltd, R&D Synthet Biol, Navi Mumbai, India
Bandyopadhyay, Anindya
;
Kancharla, Nagesh
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h-index: 0
机构:
Reliance Ind Ltd, R&D Synthet Biol, Navi Mumbai, IndiaReliance Ind Ltd, R&D Synthet Biol, Navi Mumbai, India
Kancharla, Nagesh
;
Javalkote, Vivek S.
论文数: 0引用数: 0
h-index: 0
机构:
Reliance Ind Ltd, R&D Synthet Biol, Navi Mumbai, IndiaReliance Ind Ltd, R&D Synthet Biol, Navi Mumbai, India
Javalkote, Vivek S.
;
Dasgupta, Santanu
论文数: 0引用数: 0
h-index: 0
机构:
Reliance Ind Ltd, R&D Synthet Biol, Navi Mumbai, IndiaReliance Ind Ltd, R&D Synthet Biol, Navi Mumbai, India
Dasgupta, Santanu
;
Brutnell, Thomas P.
论文数: 0引用数: 0
h-index: 0
机构:
Chinese Acad Agr Sci, Biotechnol Res Inst, Beijing, Peoples R China
Gateway Biotechnol Inc, St Louis, MO USAReliance Ind Ltd, R&D Synthet Biol, Navi Mumbai, India
机构:
Rockefeller Univ, Bacteriol Lab, New York, NY 10065 USARockefeller Univ, Bacteriol Lab, New York, NY 10065 USA
Bikard, David
;
Jiang, Wenyan
论文数: 0引用数: 0
h-index: 0
机构:
Rockefeller Univ, Bacteriol Lab, New York, NY 10065 USARockefeller Univ, Bacteriol Lab, New York, NY 10065 USA
Jiang, Wenyan
;
Samai, Poulami
论文数: 0引用数: 0
h-index: 0
机构:
Rockefeller Univ, Bacteriol Lab, New York, NY 10065 USARockefeller Univ, Bacteriol Lab, New York, NY 10065 USA
Samai, Poulami
;
Hochschild, Ann
论文数: 0引用数: 0
h-index: 0
机构:
Harvard Univ, Sch Med, Dept Microbiol & Immunobiol, Boston, MA 02115 USARockefeller Univ, Bacteriol Lab, New York, NY 10065 USA
Hochschild, Ann
;
Zhang, Feng
论文数: 0引用数: 0
h-index: 0
机构:
Broad Inst MIT & Harvard, Cambridge, MA 02142 USA
MIT, McGovern Inst Brain Res, Cambridge, MA 02139 USA
MIT, Dept Brain & Cognit Sci, Cambridge, MA 02139 USA
MIT, Dept Biol Engn, Cambridge, MA 02139 USARockefeller Univ, Bacteriol Lab, New York, NY 10065 USA
Zhang, Feng
;
Marraffini, Luciano A.
论文数: 0引用数: 0
h-index: 0
机构:
Rockefeller Univ, Bacteriol Lab, New York, NY 10065 USARockefeller Univ, Bacteriol Lab, New York, NY 10065 USA
机构:
USDA ARS, Vegetable Crops Res Unit, Madison, WI 53726 USA
Univ Wisconsin, Dept Hort, 1575 Linden Dr, Madison, WI 53706 USAUSDA ARS, Vegetable Crops Res Unit, Madison, WI 53726 USA
Butler, Nathaniel M.
;
Jansky, Shelley H.
论文数: 0引用数: 0
h-index: 0
机构:
USDA ARS, Vegetable Crops Res Unit, Madison, WI 53726 USA
Univ Wisconsin, Dept Hort, 1575 Linden Dr, Madison, WI 53706 USAUSDA ARS, Vegetable Crops Res Unit, Madison, WI 53726 USA
Jansky, Shelley H.
;
Jiang, Jiming
论文数: 0引用数: 0
h-index: 0
机构:
Michigan State Univ, Dept Hort, Dept Plant Biol, E Lansing, MI 48824 USAUSDA ARS, Vegetable Crops Res Unit, Madison, WI 53726 USA
机构:
Michigan State Univ, Dept Plant Soils & Microbial Sci, E Lansing, MI 48824 USAMichigan State Univ, Dept Plant Soils & Microbial Sci, E Lansing, MI 48824 USA
Butler, Nathaniel M.
;
Baltes, Nicholas J.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Minnesota, Dept Genet Cell Biol & Dev, Minneapolis, MN USA
Univ Minnesota, Ctr Genome Engn, Minneapolis, MN USAMichigan State Univ, Dept Plant Soils & Microbial Sci, E Lansing, MI 48824 USA
Baltes, Nicholas J.
;
Voytas, Daniel F.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Minnesota, Dept Genet Cell Biol & Dev, Minneapolis, MN USA
Univ Minnesota, Ctr Genome Engn, Minneapolis, MN USAMichigan State Univ, Dept Plant Soils & Microbial Sci, E Lansing, MI 48824 USA
Voytas, Daniel F.
;
Douches, David S.
论文数: 0引用数: 0
h-index: 0
机构:
Michigan State Univ, Dept Plant Soils & Microbial Sci, E Lansing, MI 48824 USAMichigan State Univ, Dept Plant Soils & Microbial Sci, E Lansing, MI 48824 USA