The uplift of the Tibetan Plateau significantly affected the global climate system. However, the timing of its uplift and the formation of its vast expanse are poorly understood. The occurrence of two types of leucogranites (the two-mica leucogranites and garnet-bearing leucogranites) identified in the Ailaoshan-Red River (ASRR) shear zone suggests an extension event in the southeastern Tibetan Plateau. The age of these leucogranites could be used to constrain the timing of uplift and southeastward expansion of the plateau. Petrography, geochronology and geochemistry investigations, including Sr-Nd isotope analysis, were conducted on the two-mica leucogranites and garnet-bearing leucogranites from the ASRR shear zone. LA-ICP-MS zircon U-Pb dating indicates that these rocks were emplaced at similar to 27 Ma, implying that the Tibetan Plateau had already achieved maximum uplift prior to the late Oligocene. It subsequently started to expand southeastward as a result of crustal flow. Compared to classic metapelite-derived leucogranites from Himalaya, the two-mica leucogranites show high K2O/Na2O (1.31-1.92), low Rb/Sr, CaO, lower Sr-87/Sr-86 ratios (0.7089-0.7164) and higher epsilon(Nd)(t) (-8.83 to -3.10). This whole-rock geochemical characteristics likely indicates a mixing source origin, composed predominantly of amphibolite with subordinated metapelite, which is also evidenced by Sr-87/Sr-86 vs. epsilon(Nd)(t) diagram. However, The garnet-bearing leucogranites with high SiO2 contents (72.25-74.12 wt%) have high initial Sr-87/Sr-86 ratios (0.7332-0.7535) and low epsilon(Nd)(t) (-16.36 to -18.98), indicating that they are derived from the source comprised of metapelite and results of fluexed muscovite melting under lower crustal level, which is also evidenced by the Rb-Sr-Ba systematics. These leucogranites formed from partial melting of the thickened lower crust, which resulted in the formation of granitic melt that weakened the crust. The weakened crust aided the left-lateral strike-slip movement of the ASRR shear zone, triggering the escape of the Indochina terrane in the southeastern Tibetan Plateau during the late Oligocene.