Structural basis for recognition of transcriptional terminator structures by ProQ/FinO domain RNA chaperones

被引:5
作者
Kim, Hyeong Jin [1 ]
Black, Mazzen [1 ]
Edwards, Ross A. [1 ]
Peillard-Fiorente, Flora [2 ]
Panigrahi, Rashmi [1 ]
Klinger, David [3 ]
Eidelpes, Reiner [3 ]
Zeindl, Ricarda [3 ]
Peng, Shiyun [1 ]
Su, Jikun [1 ]
Omar, Ayat R. [1 ]
MacMillan, Andrew M. [1 ]
Kreutz, Christoph [3 ]
Tollinger, Martin [3 ]
Charpentier, Xavier [2 ]
Attaiech, Laetitia [2 ]
Glover, J. N. Mark [1 ]
机构
[1] Univ Alberta, Dept Biochem, Edmonton, AB T6G 2H7, Canada
[2] Univ Claude Bernard Lyon 1, Univ Lyon,Ecole Normale Super Lyon, Team Horizontal Gene Transfer Bacterial Pathogens, Ctr Int Rech Infectiol,Inserm,U1111,CNRS,UMR5308, F-69100 Villeurbanne, France
[3] Univ Innsbruck, Ctr Mol Biosci Innsbruck CMBI, Inst Organ Chem, Nnrain 80-82, A-6020 Innsbruck, Austria
基金
加拿大健康研究院; 奥地利科学基金会; 加拿大自然科学与工程研究理事会;
关键词
FINP ANTISENSE RNA; MESSENGER-RNA; PROTEIN; FAMILY; REPRESSOR; FEATURES; PROQ; HFQ;
D O I
10.1038/s41467-022-34875-5
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
The ProQ/FinO family of RNA binding proteins mediate sRNA-directed gene regulation throughout gram-negative bacteria. Here, we investigate the structural basis for RNA recognition by ProQ/FinO proteins, through the crystal structure of the ProQ/FinO domain of the Legionella pneumophila DNA uptake regulator, RocC, bound to the transcriptional terminator of its primary partner, the sRNA RocR. The structure reveals specific recognition of the 3' nucleotide of the terminator by a conserved pocket involving a beta-turn-alpha-helix motif, while the hairpin portion of the terminator is recognized by a conserved alpha-helical N-cap motif. Structure-guided mutagenesis reveals key RNA contact residues that are critical for RocC/RocR to repress the uptake of environmental DNA in L. pneumophila. Structural analysis and RNA binding studies reveal that other ProQ/FinO domains also recognize related transcriptional terminators with different specificities for the length of the 3' ssRNA tail. In this work, the authors determine the crystal structure of a ProQ/FinO RNA chaperone bound to its RNA target. This provides insight into how this family of bacterial proteins recognize transcriptional terminator structures.
引用
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页数:12
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