Nanocarriers Made of Proteins: Intracellular Visualization of a Smart Biodegradable Drug Delivery System

被引:6
|
作者
Frey, Marie-Luise [1 ]
Han, Shen [1 ]
Halim, Henry [1 ]
Kaltbeitzel, Anke [1 ]
Riedinger, Andreas [1 ]
Landfester, Katharina [1 ]
Lieberwirth, Ingo [1 ]
机构
[1] Max Planck Inst Polymer Res, Ackermannweg 10, D-55128 Mainz, Germany
关键词
correlative microscopy; drug delivery; intracellular pathways; protein nanocarriers; transmission electron microscopy; QUANTUM DOTS; NANOCAPSULES;
D O I
10.1002/smll.202106094
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
This work analyzes the intracellular fate of protein-based nanocarriers along their endolysosomal pathway by means of correlative light and electron microscopy methods. To unambiguously identify the nanocarriers and their degradation remnants in the cellular environment, they are labeled with fluorescent, inorganic nanoplatelets. This allows tracking the nanocarriers on their intracellular pathway by means of electron microscopy imaging. From the present data, it is possible to identify different cell compartments in which the nanocarriers are processed. Finally, three different terminal routes for the intracellular destiny of the nanocarriers are presented. These findings are important to reveal the degradation process of protein nanocapsules and contribute to the understanding of the therapeutic success of an encapsulated drug.
引用
收藏
页数:8
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