Recovery and cryopreservation of epididymal sperm from domestic cat using powdered coconut water (ACP-117c) and TRIS extenders

被引:5
|
作者
Barbosa, Brenna de Sousa [1 ]
Izzo, Roberta Goncalves [2 ]
Rodrigues Silva, Herlon Victor [1 ]
Pereira Nunes, Thalles Gothardo [1 ]
Brito, Bruna Farias [1 ]
Pereira da Silva, Ticiana Franco [1 ]
Machado da Silva, Lucia Daniel [1 ]
机构
[1] Univ Estadual Ceara, Sch Vet Med, Fortaleza, CE, Brazil
[2] Fed Rural Univ Semiarid, Sch Vet Med, Mossoro, RN, Brazil
关键词
Felines; Alternative extender; Conservation; Spermatozoa; SPERMATOZOA; FERTILITY; MEMBRANE; STORAGE; PROTOCOLS; INTEGRITY; BANKING; IMPACT; CANINE;
D O I
10.1016/j.cryobiol.2019.11.042
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
Cryopreservation of cats epididymal spermatozoa allows the conservation of the genetic material and the study of the cryogenic effect applied to the gametes of other felines. However, thisbiotechnique still presents variable results, being necessary the investigation of alternative extenders. Powdered coconut water (ACP-117c) has been efficient in the sperm freezing of several species and in the cat sperm refrigeration. Therefore, we aimed to evaluate the effect of the freezing stages and the quality of the cats' epididymal spermatozoa after thawing, using ACP-117c. Epididymides (n = 36) from 18 cats were processed using TRIS (n = 18) or ACP-117c (n = 18) for sperm recovery. The sperm were immediately evaluated. Then, this was cooled, glycerolized, frozen and thawed, and re-evaluated at each stage for sperm kinetics by Computer Assisted Semen Analysis, viability, functionality (HOST), mitochondrial activity (DAB) and morphology. There was a reduction in total motility and progressive motility after thawing in both groups, and TRIS was superior to ACP-117c. The curvilinear velocity reduced after thawing with ACP-117c. Viability decreased after glycerolization in TRIS. Although it also reduced after thawing in both groups, it was higher in TRIS. There was no change on HOST. Mitochondrial activity decreased during the cryopreservation steps for both extenders. Nevertheless, TRIS presented a higher percentage of spermatozoa from DAB class I and II after thawing. Morphology did not differ between extenders. Therefore, ACP-117c is an alternative for the recovery of cat epididymal spermatozoa; however, it is not efficient for freezing. Glycerolization and thawing are the most critical stages, regardless of the extender.
引用
收藏
页码:103 / 108
页数:6
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