His-tag based in situ labelling of progeny viruses for real-time single virus tracking in living cells

被引：13

作者：

Zheng, Lin Ling ^{[1
]}

Li, Chun Mei ^{[1
]}

Zhen, Shu Jun ^{[2
]}

Li, Yuan Fang ^{[2
]}

Huang, Cheng Zhi ^{[1
,2
]}

机构：

[1] Southwest Univ, Coll Pharmaceut Sci, Minist Educ, Key Lab Luminescent & Real Time Analyt Chem, Chongqing 400715, Peoples R China

[2] Southwest Univ, Coll Chem & Chem Engn, Chongqing Sci & Technol Commiss, Chongqing Key Lab Biomed Anal, Chongqing 400715, Peoples R China

来源：

NANOSCALE | 2016年 / 8卷 / 44期

基金：

中国博士后科学基金; 中国国家自然科学基金;

关键词：

CLATHRIN-MEDIATED ENDOCYTOSIS; QUANTUM DOTS; LIVE CELLS; ENVELOPED VIRUSES; HOST-CELLS; PROTEINS; CHEMISTRY; ENTRY; NANOPARTICLES; PURIFICATION;

D O I：

10.1039/c6nr05806j

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Tracking virus infection events in live cells is useful for understanding the mechanism of virus infection, and fluorescent labelling is a critical step. Herein a noninvasive strategy for labelling viruses with His-tags was developed by in situ modifying the cell surface proteins with polypeptides containing His-tags during progeny virus assembly. The His-tagged viruses were further conjugated with Ni2+-nitrilotriacetate complex modified quantum dots, and retained their infectivity for real-time single virus tracking in living cells.

引用

页码：18635 / 18639

页数：5

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