His-tag based in situ labelling of progeny viruses for real-time single virus tracking in living cells

被引：13
|
作者：
Zheng, Lin Ling [1 ]
Li, Chun Mei [1 ]
Zhen, Shu Jun [2 ]
Li, Yuan Fang [2 ]
Huang, Cheng Zhi [1 ,2 ]
机构：
[1] Southwest Univ, Coll Pharmaceut Sci, Minist Educ, Key Lab Luminescent & Real Time Analyt Chem, Chongqing 400715, Peoples R China
[2] Southwest Univ, Coll Chem & Chem Engn, Chongqing Sci & Technol Commiss, Chongqing Key Lab Biomed Anal, Chongqing 400715, Peoples R China
来源：
NANOSCALE | 2016年 / 8卷 / 44期
基金：
中国博士后科学基金; 中国国家自然科学基金;
关键词：
CLATHRIN-MEDIATED ENDOCYTOSIS; QUANTUM DOTS; LIVE CELLS; ENVELOPED VIRUSES; HOST-CELLS; PROTEINS; CHEMISTRY; ENTRY; NANOPARTICLES; PURIFICATION;
D O I：
10.1039/c6nr05806j
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
Tracking virus infection events in live cells is useful for understanding the mechanism of virus infection, and fluorescent labelling is a critical step. Herein a noninvasive strategy for labelling viruses with His-tags was developed by in situ modifying the cell surface proteins with polypeptides containing His-tags during progeny virus assembly. The His-tagged viruses were further conjugated with Ni2+-nitrilotriacetate complex modified quantum dots, and retained their infectivity for real-time single virus tracking in living cells.
引用
收藏
页码：18635 / 18639
页数：5
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