Insertion of excised IgH switch sequences causes overexpression of cyclin D1 in a myeloma tumor cell

被引：80

作者：

Gabrea, A

Bergsagel, PL

Chesi, M

Shou, YP

Kuehl, WM ^{[1
]}

机构：

[1] NCI, Dept Genet, Med Branch, Bethesda, MD 20889 USA

[2] Cornell Univ, Weill Med Coll, Div Hematol & Oncol, Dept Med, New York, NY 10021 USA

来源：

MOLECULAR CELL | 1999年 / 3卷 / 01期

关键词：

D O I：

10.1016/S1097-2765(00)80180-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Oncogenes are often dysregulated in B cell tumors as a result of a reciprocal translocation involving an immunoglobulin locus. The translocations are caused by errors in two developmentally regulated DNA recombination processes: V(D)J and IgH switch recombination. Both processes share the property of joining discontinuous sequences from one chromosome and releasing intervening sequences as circles that are lost from progeny cells. Here we show that these intervening sequences may instead insert in the genome and that during productive IgH mu-epsilon switch recombination in U266 myeloma tumor cells, a portion of the excised IgH switch intervening sequences containing the 3' alpha-1 enhancer has inserted on chromosome 11q13, resulting in overexpression of the adjacent cyclin D1 oncogene.

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页码：119 / 123

页数：5

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