Identification of DNA-PK in the arthropods -: Evidence for the ancient ancestry of vertebrate non-homologous end-joining

被引：17

作者：

Doré, AS ^{[1
]}

Drake, ACB ^{[1
]}

Brewerton, SC ^{[1
]}

Blundell, TL ^{[1
]}

机构：

[1] Univ Cambridge, Dept Biochem, Cambridge CB2 1GA, England

来源：

DNA REPAIR | 2004年 / 3卷 / 01期

基金：

英国生物技术与生命科学研究理事会;

关键词：

DNA-PKcs; Ku; Anopheles gambiae; Drosophila; NHEJ; DNA repair; genomics;

D O I：

10.1016/j.dnarep.2003.09.003

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Cellular life depends upon the preservation and transmission of genetic material. Double stranded DNA breaks (DSBs) cause catastrophic gene loss in cell division and must be promptly and accurately repaired. In eukaryotes DSBs may be repaired by either non-homologous end-joining (NHEJ), single strand annealing or homologous recombination (HR). Vertebrate NHEJ has been shown to depend upon the DNA-dependent protein kinase (DNA-PK) consisting of the phosphatidylinositol 3 (PI3)-kinase like (PIKK) catalytic sub-unit (DNA-PKcs) and the DNA targeting factor Ku. Our analysis of recently completed genomes found several novel PIKKs in Anopheles gambiae and Drosophila melanogaster including a novel mosquito DNA-PKcs orthologue, the first non-vertebrate DNA-PKcs described to date. We also detected a DNA-PKcs fragment in the high quality EST set of Apis mellifera ligustica (honey bee) suggesting that DNA-PK is a far older and more important eukaryotic complex than previously thought. (C) 2003 Elsevier B.V. All rights reserved.

引用

页码：33 / 41

页数：9

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