Slow freezing versus vitrification technique for human ovarian tissue cryopreservation: An evaluation of histological changes, WNT signaling pathway and apoptotic genes expression L

被引:36
作者
Dalman, Azam [1 ]
Farahani, Nafiseh Sadat Deheshkar Gooneh [1 ]
Totonchi, Mehdi [2 ]
Pirjani, Reihaneh [3 ]
Ebrahimi, Bita [1 ]
Valojerdi, Mojtaba Rezazadeh [1 ,4 ]
机构
[1] ACECR, Royan Inst Reprod Biomed, Dept Embryol, Reprod Biomed Res Ctr, POB 19395-4644, Tehran, Iran
[2] ACECR, Dept Genet, Reprod Biomed Res Ctr, Royan Inst Reprod Biomed, Tehran, Iran
[3] Univ Tehran Med Sci, Arash Hosp, Dept Obstet & Gynecol, Tehran, Iran
[4] Tarbiat Modares Univ, Dept Anat, Fac Med Sci, POB 14115-111, Tehran, Iran
关键词
Apoptotic genes; Human ovarian tissue; Vitrification; Slow freezing; WNT signaling; BETA-CATENIN; GRANULOSA-CELLS; BOVINE; TRANSPLANTATION; FERTILITY;
D O I
10.1016/j.cryobiol.2017.09.007
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
This study compared slow freezing and vitrification of ovarian tissue by evaluation of histological changes, WNT signaling pathway and apoptotic genes expression. Ovarian tissue was obtained from women aging 27-38 years old. Ovarian cortex from each patient was divided into three pieces and randomly grouped as slow freezing, vitrification and control groups for investigation of WNT signaling gene expression and beta-CATENIN presence as well as histological studies. The stromal structure of all ovaries were preserved. The number of secondary follicles decreased in vitrified group (P < 0.05). WNT-3, beta-CATENIN, FZD-2 and GSIC-3 beta expressions were significantly higher in slow frozen and vitrified groups, compared to control group (P < 0.05). On the contrary, AXIN1 expression in slow frozen samples were significantly lower than that of the vitrified and control group. The expression of apoptotic genes, excluding CASP3, was significantly decreased in slow-frozen samples (P < 0.05). Conversely, BAX:BCL-2 percentage significantly increased in vitrification versus slow freezing and control(P < 0.05). Follicles in slow frozen samples displayed nuclear and cytoplasmic beta-CATENIN staining, while control and vitrification groups only showed beta-CATENIN protein in the cytoplasm. The presented data show that slow freezing results in a better preservation regardless of the type of follicle. Therefore, it is concluded that slow freezing is still an ideal method for ovary cryopreservation. (C) 2017 Elsevier Inc. All rights reserved.
引用
收藏
页码:29 / 36
页数:8
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