A multiplex PCR for improved detection of typical and atypical BCR-ABL fusion transcripts

被引：60

作者：

Burmeister, Thomas ^{[1
]}

Reinhardt, Richard ^{[2
]}

机构：

[1] Charite, Med Klin 3, D-12200 Berlin, Germany

[2] Max Planck Inst Mol Genet, D-14195 Berlin, Germany

来源：

LEUKEMIA RESEARCH | 2008年 / 32卷 / 04期

关键词：

BCR-ABL fusion proteins; myeloproliferative disorders; chronic myeloid leukemia; acute lymphoblastic leukemia; polymerase chain reaction;

D O I：

10.1016/j.leukres.2007.08.017

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

RT-PCR is the method of choice for detecting BCR-ABL in CML and ALL. The three predominant mRNA transcripts found are e1a2 (in ALL), e13a2, and e14a2 (in CML and ALL). However, a number of "atypical" BCR-ABL transcripts (e1a3, e13a3, e14a3, e19a2, e6a2, e8a2, etc.) resulting from chromosomal breakpoints outside ABL intron 1 or BCR intron 1, 13 or 14, respectively, have been reported. These atypical transcripts may escape detection when using methods that are optimized to detect just the typical ones. We present here a novel, fast, and reliable multiplex PCR for improved detection of typical and atypical BCR-ABL transcripts. (C) 2007 Elsevier Ltd. All rights reserved.

引用

页码：579 / 585

页数：7

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