Molecular Detection of Campylobacter spp. in California Gull (Larus californicus) Excreta

被引：30

作者：

Lu, Jingrang ^{[1
]}

Ryu, Hodon ^{[2
]}

Domingo, Jorge W. Santo ^{[2
]}

Griffith, John F. ^{[3
]}

Ashbolt, Nicholas ^{[1
]}

机构：

[1] US EPA, Natl Exposure Res Lab, Cincinnati, OH 45268 USA

[2] US EPA, Natl Risk Management Res Lab, Cincinnati, OH 45268 USA

[3] So Calif Coastal Water Res Project, Costa Mesa, CA USA

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2011年 / 77卷 / 14期

关键词：

PCR ASSAY; WATER; JEJUNI; IDENTIFICATION; EPIDEMIOLOGY; DIVERSITY; BACTERIA; GENE; DELAWARENSIS; PREVALENCE;

D O I：

10.1128/AEM.00018-11

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We examined the prevalence, quantity, and diversity of Campylobacter species in the excreta of 159 California gull (Larus californicus) samples using culture-, PCR-, and quantitative PCR (qPCR)-based detection assays. Campylobacter prevalence and abundance were relatively high in the gull excreta examined; however, C. jejuni and C. lari were detected in fewer than 2% of the isolates and DNA extracts from the fecal samples that tested positive. Moreover, molecular and sequencing data indicated that most L. californicus campylobacters were novel (<97% 16S rRNA gene sequence identity to known Campylobacter species) and not closely related to species commonly associated with human illness. Campylobacter estimates were positively related with those of fecal indicators, including a gull fecal marker based on the Catellicoccus marimammalium 16S rRNA gene.

引用

页码：5034 / 5039

页数：6

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