Bioluminescence imaging of dual gene expression at the single-cell level

被引：26

作者：

Kwon, HyuckJoon

Enomoto, Toshiteru ^{[2
]}

Shimogawara, Masahiro ^{[2
]}

Yasuda, Kazunori

Nakajima, Yoshihiro ^{[3
]}

Ohmiya, Yoshihiro ^{[1
,4
]}

机构：

[1] Hokkaido Univ, Grad Sch Med, Res Ctr Cooperat Projects, Regenerat Med Tissue Engn Div,Kita Ku, Sapporo, Hokkaido 0608638, Japan

[2] ATTO Corp, Tokyo, Japan

[3] Natl Inst Adv Ind Sci & Technol, Hlth Res Inst, Ikeda, Osaka, Japan

[4] Natl Inst Adv Ind Sci & Technol, Bioprod Res Inst, Tsukuba, Ibaraki, Japan

来源：

BIOTECHNIQUES | 2010年 / 48卷 / 06期

关键词：

bioluminescence; gene expression; multicolored luciferase; single-cell imaging;

D O I：

10.2144/000113419

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Bioluminescence imaging reveals the long-term dynamics of individual gene expression in a single cell. However, methods for simultaneously imaging multiple gene expression patterns have been unknown to date. Here, we constructed a dual-path optical luminescence imaging system using a two-color reporter system and could simultaneously track two gene expression patterns for several days in a single cell.

引用

页码：460 / 462

页数：3

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