Identification of a novel Hemoplasma species from pigs in Zhejiang province, China

被引：21

作者：

Fu, Yuan ^{[1
,2
]}

Shi, Tuanyuan ^{[2
]}

Xu, Lihua ^{[2
]}

Wei, Wei ^{[2
,3
]}

Lu, Fuzhuang ^{[2
]}

Zhang, Xuejuan ^{[2
]}

Yuan, Xiufang ^{[2
]}

Li, Junxing ^{[2
]}

Lv, Jinhui ^{[2
]}

Fang, Weihuan ^{[1
]}

机构：

[1] Zhejiang Univ, Inst Prevent Vet Med, Hangzhou 310058, Zhejiang, Peoples R China

[2] Zhejiang Acad Agr Sci, Inst Anim Husb & Vet Med, Hangzhou 310021, Zhejiang, Peoples R China

[3] Southwest Univ Nationalities, Coll Life Sci & Technol, Chengdu 610041, Sichuan, Peoples R China

来源：

JOURNAL OF VETERINARY MEDICAL SCIENCE | 2017年 / 79卷 / 05期

关键词：

hemoplasma; Mycoplasma suis; Mycoplasma parvum; novel hemotropic Mycoplasma hemosuis; 16S RIBOSOMAL-RNA; MOLECULAR CHARACTERIZATION; RISK-FACTORS; ASSAY; PCR; MYCOPLASMAS; CATS; GENE;

D O I：

10.1292/jvms.16-0545

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

Hemoplasmas belong to Mycoplasmataceae (Mollicutes: Mycoplasmatales) and are able to infect a broad range of mammalian species. We investigated prevalence of hemotropic mycoplasma species in pig farms in the region of Zhejiang by a PCR scheme using universal primers targeting 16S rRNA and RNase P RNA gene (rnpB). Representative positive samples from different farms were selected for sequencing of 16S rRNA and the 219bp rnpB gene fragments for phylogenetic analysis. Sequencing analysis of PCR products from first samples identified a novel hemoplasma species present in several pig farms in the region with highest nucleotide identity of 92% to Candidatus Mycoplasma turicensis. A duplex PCR assay was then designed for differential detection of the novel hemoplasma from Mycoplasma parvum/M. suis in field samples. Of 324 blood samples from clinically healthy pigs, 26.5% was positive for this novel hemoplasma species and 50% positive for M. suis/M. parvum, indicating that the novel hemotropic mycoplasma species were of considerably high prevalence in Zhejiang province, China.

引用

页码：864 / 870

页数：7

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