Graft Versus Leukemia Response Without Graft-versus-host Disease Elicited By Adoptively Transferred Multivirus-specific T-cells

被引：25

作者：

Melenhorst, Jan J. ^{[1
,6
]}

Castillo, Paul ^{[2
,3
]}

Hanley, Patrick J. ^{[4
,5
]}

Keller, Michael D. ^{[4
,5
]}

Krance, Robert A. ^{[2
,3
]}

Margolin, Judith ^{[2
,3
]}

Leen, Ann M. ^{[2
,3
]}

Heslop, Helen E. ^{[2
,3
]}

Barrett, A. John ^{[1
]}

Rooney, Cliona M. ^{[2
,3
]}

Bollard, Catherine M. ^{[2
,3
,4
,5
]}

机构：

[1] NHLBI, Hematol Branch, NIH, Bethesda, MD 20892 USA

[2] Texas Childrens Hosp, Baylor Coll Med, Ctr Cell & Gene Therapy, Houston, TX 77030 USA

[3] Houston Methodist Hosp, Houston, TX USA

[4] Childrens Natl Hlth Syst, Program Cell Enhancement & Technol Immunotherapy, Sheikh Zayed Inst Pediat Surg Innovat, Washington, DC USA

[5] Childrens Natl Hlth Syst, Ctr Canc & Immunol Res, Washington, DC USA

[6] Univ Penn, Translat Res Program, Perelman Sch Med, Philadelphia, PA 19104 USA

来源：

MOLECULAR THERAPY | 2015年 / 23卷 / 01期

关键词：

ACUTE LYMPHOBLASTIC-LEUKEMIA; BONE-MARROW TRANSPLANT; EPSTEIN-BARR-VIRUS; LYMPHOPROLIFERATIVE DISEASE; PROPHYLACTIC INFUSION; HUMAN CYTOMEGALOVIRUS; CORD BLOOD; EBV; DONOR; RECIPIENTS;

D O I：

10.1038/mt.2014.192

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A 12-year-old boy with refractory acute lymphoblastic leukemia received a haploidentical transplant from his mother. As prophylaxis for Epstein-Barr virus (EBV), cytomegalovirus (CMV) and adenovirus, he received ex vivo expanded virus-specific donor T cells 3.5 months after transplant. Four weeks later leukemic blasts bearing the E2A deletion, identified by fluorescent in situ hybridization (FISH), appeared transiently in the blood followed by a FISH-negative hematological remission, which was sustained until a testicular relapse 3.5 months later. Clearance of the circulating leukemic cells coincided with a marked increase in circulating virus-specific T cells. The virus-specific cytotoxic T-cell (CTL) line showed strong polyfunctional reactivity with the patient's leukemic cells but not phytohemagglutinin (PHA) blasts, suggesting that virus-specific CTL lines may have clinically significant antileukemia activity.

引用

页码：179 / 183

页数：5

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