Production of xylooligosaccharides from corncob xylan by fungal xylanase and their utilization by probiotics

被引：223

作者：

Chapla, Digantkumar ^{[1
]}

Pandit, Pratima ^{[1
]}

Shah, Amita ^{[1
]}

机构：

[1] Sardar Patel Univ, BRD Sch Biosci, Vallabh Vidyanagar 388120, Gujarat, India

来源：

BIORESOURCE TECHNOLOGY | 2012年 / 115卷

关键词：

Xylooligosaccharides; Aspergillus foetidus MTCC 4898; Probiotic; Corncobs; Xylanase; IN-VITRO FERMENTATION; ENZYMATIC PRODUCTION; THERMOTOGA-MARITIMA; AUTOHYDROLYSIS; OLIGOSACCHARIDES; PURIFICATION; MANUFACTURE; XYLOBIOSE;

D O I：

10.1016/j.biortech.2011.10.083

中图分类号：

S2 [农业工程];

学科分类号：

0828 ;

摘要：

The selective production of xylooligosaccharides (XOS) was carried out using partially purified xylanase from Aspergillus foetidus MTCC 4898. Corncob xylan was extracted using a mild alkali treatment which yielded 178.73 +/- 5.8 g of xylan/kg of corncobs. Partially purified beta-xylosidase free xylanase was found efficient in releasing xylooligosaccharides from corncob xylan. Maximum yield of xylooligosaccharides was 6.73 +/- 0.23 mg/ml after 8 h of reaction time using 20 U of xylanase at 45 degrees C. Purification of XOS was done using activated charcoal column chromatography. The purified XOS preparation contained mainly xylobiose and xylotriose. XOS mixture was found suitable for food industry looking at its high thermal stability at low pH. Prebiotic effect of XOS was evaluated by in vitro fermentation of XOS using known probiotic strains viz. Bifidobacterium adolescentis, Bifidobacterium bifidum, Lactobacillus fermentum, Lactobacillus acidophilus. The results of this study revealed better growth of Bifidobacteriurn spp. on XOS than Lactobacillus spp. (C) 2011 Elsevier Ltd. All rights reserved.

引用

页码：215 / 221

页数：7

共 32 条

[21] SACCHARIFICATION OF AGRO-WASTE MATERIALS BY FUNGAL CELLULASES AND HEMICELLULASES
OKEKE, BC
OBI, SKC
[J]. BIORESOURCE TECHNOLOGY, 1995, 51 (01) : 23 - 27
[22] Production of xylooligosaccharides by autohydrolysis of lignocellulosic materials
Parajó, JC
Garrote, G
Cruz, JM
Dominguez, H
[J]. TRENDS IN FOOD SCIENCE & TECHNOLOGY, 2004, 15 (3-4) : 115 - 120
[23] Preparation of (1 → 4)-β-D-xylooligosaccharides from an acid hydrolysate of cotton-seed xylan:: suitability of cotton-seed xylan as a starting material for the preparation of (1 → 4)-β-D-xylooligosaccharides
Sun, HJ
Yoshida, S
Park, NH
Kusakabe, I
[J]. CARBOHYDRATE RESEARCH, 2002, 337 (07) : 657 - 661
[24] Production of xylobiose from the autohydrolysis explosion liquor of corncob using Thermotoga maritima xylanase B (XynB) immobilized on nickel-chelated Eupergit C
Tan, S. S.
Li, D. Y.
Jiang, Z. Q.
Zhu, Y. P.
Shi, B.
Li, L. T.
[J]. BIORESOURCE TECHNOLOGY, 2008, 99 (01) : 200 - 204
[25] Production of xylooligosaccharides from the steam explosion liquor of corncobs coupled with enzymatic hydrolysis using a thermostable xylanase
Teng, Chao
Yan, Qiaojuan
Jiang, Zhengqiang
Fan, Guangsen
Shi, Bo
[J]. BIORESOURCE TECHNOLOGY, 2010, 101 (19) : 7679 - 7682
[26] Enzymatic processing of crude, xylooligomer solutions obtained by autohydrolysis of Eucalyptus wood
Vázquez, MJ
Alonso, JL
Domínguez, H
Parajó, JC
[J]. FOOD BIOTECHNOLOGY, 2002, 16 (02) : 91 - 105
[27] Xylooligosaccharides:: manufacture and applications
Vázquez, MJ
Alonso, JL
Domínguez, H
Parajó, JC
[J]. TRENDS IN FOOD SCIENCE & TECHNOLOGY, 2000, 11 (11) : 387 - 393
[28] In vitro fermentation of xylooligosaccharides from wheat bran insoluble dietary fiber by Bifidobacteria
Wang, Jing
Sun, Baoguo
Cao, Yanping
Wang, Chengtao
[J]. CARBOHYDRATE POLYMERS, 2010, 82 (02) : 419 - 423
[29] Enzymatic preparation of wheat bran xylooligosaccharides and their stability during pasteurization and autoclave sterilization at low pH
Wang, Jing
Sun, Baoguo
Cao, Yanping
Tian, Yuan
[J]. CARBOHYDRATE POLYMERS, 2009, 77 (04) : 816 - 821
[30] Aqueous extraction of corncob xylan and production of xylooligosaccharides
Yang, R
Xu, S
Wang, Z
Yang, W
[J]. LWT-FOOD SCIENCE AND TECHNOLOGY, 2005, 38 (06) : 677 - 682

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