Production of xylooligosaccharides from corncob xylan by fungal xylanase and their utilization by probiotics

被引：223

作者：

Chapla, Digantkumar ^{[1
]}

Pandit, Pratima ^{[1
]}

Shah, Amita ^{[1
]}

机构：

[1] Sardar Patel Univ, BRD Sch Biosci, Vallabh Vidyanagar 388120, Gujarat, India

来源：

BIORESOURCE TECHNOLOGY | 2012年 / 115卷

关键词：

Xylooligosaccharides; Aspergillus foetidus MTCC 4898; Probiotic; Corncobs; Xylanase; IN-VITRO FERMENTATION; ENZYMATIC PRODUCTION; THERMOTOGA-MARITIMA; AUTOHYDROLYSIS; OLIGOSACCHARIDES; PURIFICATION; MANUFACTURE; XYLOBIOSE;

D O I：

10.1016/j.biortech.2011.10.083

中图分类号：

S2 [农业工程];

学科分类号：

0828 ;

摘要：

The selective production of xylooligosaccharides (XOS) was carried out using partially purified xylanase from Aspergillus foetidus MTCC 4898. Corncob xylan was extracted using a mild alkali treatment which yielded 178.73 +/- 5.8 g of xylan/kg of corncobs. Partially purified beta-xylosidase free xylanase was found efficient in releasing xylooligosaccharides from corncob xylan. Maximum yield of xylooligosaccharides was 6.73 +/- 0.23 mg/ml after 8 h of reaction time using 20 U of xylanase at 45 degrees C. Purification of XOS was done using activated charcoal column chromatography. The purified XOS preparation contained mainly xylobiose and xylotriose. XOS mixture was found suitable for food industry looking at its high thermal stability at low pH. Prebiotic effect of XOS was evaluated by in vitro fermentation of XOS using known probiotic strains viz. Bifidobacterium adolescentis, Bifidobacterium bifidum, Lactobacillus fermentum, Lactobacillus acidophilus. The results of this study revealed better growth of Bifidobacteriurn spp. on XOS than Lactobacillus spp. (C) 2011 Elsevier Ltd. All rights reserved.

引用

页码：215 / 221

页数：7

共 32 条

[11] Purification and characterization of a xylanase from Trichoderma longibrachiatum for xylooligosaccharide production
Chen, C
Chen, JL
Lin, TY
[J]. ENZYME AND MICROBIAL TECHNOLOGY, 1997, 21 (02) : 91 - 96
[12] A COLORIMETRIC METHOD FOR THE DETERMINATION OF SUGARS
DUBOIS, M
GILLES, K
HAMILTON, JK
REBERS, PA
SMITH, F
[J]. NATURE, 1951, 168 (4265) : 167 - 167
[13] Potential application of yeast cellulase-free xylanase in agrowaste material treatment to remove hemicellulose fractions
Gokhale, DV
Patil, SG
Bastawde, KB
[J]. BIORESOURCE TECHNOLOGY, 1998, 63 (02) : 187 - 191
[14] The recombinant xylanase B of Thermotoga maritima is highly xylan specific and produces exclusively xylobiose from xylans, a unique character for industrial applications
Jiang, ZQ
Deng, W
Zhu, YP
Li, LT
Sheng, YJ
Hayashi, K
[J]. JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC, 2004, 27 (4-6) : 207 - 213
[15] Lowry O.H., 1951, J BIOL CHEM, V31, P426
[16] Structural characterisation and determination of prebiotic activity of purified xylo-oligosaccharides obtained from Bengal gram husk (Cicer arietinum L.) and wheat bran (Triticum aestivum)
Madhukumar, M. S.
Muralikrishna, G.
[J]. FOOD CHEMISTRY, 2010, 118 (02) : 215 - 223
[17] USE OF DINITROSALICYLIC ACID REAGENT FOR DETERMINATION OF REDUCING SUGAR
MILLER, GL
[J]. ANALYTICAL CHEMISTRY, 1959, 31 (03) : 426 - 428
[18] ASSESSMENT OF THE ACTIVITY OF A FRUCTO-OLIGOSACCHARIDE ON DIFFERENT CECAL PARAMETERS IN RABBITS EXPERIMENTALLY INFECTED WITH ESCHERICHIA-COLI 0.103
MORISSE, JP
MAURICE, R
BOILLETOT, E
COTTE, JP
[J]. ANNALES DE ZOOTECHNIE, 1993, 42 (01): : 81 - 87
[19] In vitro fermentation of xylo-oligosaccharides from corn cobs autohydrolysis by Bifidobacterium and Lactobacillus strains
Moura, Patricia
Barata, Rosario
Carvalheiro, Florbela
Girio, Francisco
Loureiro-Dias, Maria C.
Esteves, M. Paula
[J]. LWT-FOOD SCIENCE AND TECHNOLOGY, 2007, 40 (06) : 963 - 972
[20] Advances in the manufacture, purification and applications of xylo-oligosaccharides as food additives and nutraceuticals
Moure, Andres
Gullon, Patricia
Dominguez, Herminia
Parajo, Juan Carlos
[J]. PROCESS BIOCHEMISTRY, 2006, 41 (09) : 1913 - 1923

← 1 2 3 4 →