Digital Polymerase Chain Reaction in an Array of Femtoliter Polydimethylsiloxane Microreactors

被引：82

作者：

Men, Yongfan ^{[1
,2
]}

Fu, Yusi ^{[1
]}

Chen, Zitian ^{[1
,3
]}

Sims, Peter A. ^{[4
,5
]}

Greenleaf, William J. ^{[6
]}

Huang, Yanyi ^{[1
,2
,3
]}

机构：

[1] Peking Univ, Biodynam Opt Imaging Ctr BIOPIC, Sch Life Sci, Beijing 100871, Peoples R China

[2] Peking Univ, Coll Engn, Beijing 100871, Peoples R China

[3] Peking Univ, Coll Chem & Mol Engn, Beijing 100871, Peoples R China

[4] Columbia Univ, Med Ctr, Columbia Initiat Syst Biol, New York, NY 10032 USA

[5] Columbia Univ, Med Ctr, Dept Biochem & Mol Biophys, New York, NY 10032 USA

[6] Stanford Univ, Sch Med, Dept Genet, Stanford, CA 94305 USA

来源：

ANALYTICAL CHEMISTRY | 2012年 / 84卷 / 10期

基金：

中国国家自然科学基金;

关键词：

ALLELIC IMBALANCE; COUNTING ALLELES; EARLY-STAGE; PCR; MICROFLUIDICS; MUTATIONS; PLATFORM; PLASMA; BIAS;

D O I：

10.1021/ac300761n

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

We developed a simple, compact inicrofluidic device to perform high dynamic-range digital polymerase chain reaction (dPCR) in an array of isolated 36-femtoliter microreactors. The density of the microreactors exceeded 20 000/mm(2). This device, made from polydimethylsiloxane (PDMS), allows the samples to be loaded into all microreactors simultaneously. The microreactors are completely sealed through the deformation of a PM/ES membrane. The small volume of the microreactors ensures a compact device with high reaction efficiency and low reagent and sample consumption. Future potential applications of this platform include multicolor dPCR and massively parallel dPCR for next generation sequencing library preparation.

引用

页码：4262 / 4266

页数：5

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