Induction of the 'ASIA' syndrome in NZB/NZWF1 mice after injection of complete Freund's adjuvant (CFA)

被引：19

作者：

Bassi, N. ^{[1
]}

Luisetto, R. ^{[2
]}

Del Prete, D. ^{[3
]}

Ghirardello, A. ^{[1
]}

Ceol, M. ^{[3
]}

Rizzo, S. ^{[4
]}

Iaccarino, L. ^{[1
]}

Gatto, M. ^{[1
]}

Valente, M. L. ^{[4
]}

Punzi, L. ^{[1
]}

Doria, A. ^{[1
]}

机构：

[1] Univ Padua, Div Rheumatol, Dept Clin & Expt Med, I-35128 Padua, Italy

[2] Univ Padua, Dept Expt Surg, I-35128 Padua, Italy

[3] Univ Padua, Dept Med & Surg Sci, Div Nephrol, I-35128 Padua, Italy

[4] Univ Padua, Div Pathol, I-35128 Padua, Italy

来源：

LUPUS | 2012年 / 21卷 / 02期

关键词：

adjuvants; ASIA syndrome; NZB/NZWF1; mice; systemic lupus erythematosus; vaccines; SYSTEMIC-LUPUS-ERYTHEMATOSUS; AUTOIMMUNE RHEUMATIC-DISEASES; GULF-WAR SYNDROME; QUALITY-OF-LIFE; SYNTHETIC OLIGONUCLEOTIDES; NALP3; INFLAMMASOME; ITALIAN PATIENTS; BALB/C MICE; VACCINE; ANTIBODIES;

D O I：

10.1177/0961203311429553

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Adjuvants, commonly used in vaccines, may be responsible for inducing autoimmunity and autoimmune diseases, both in humans and mice. The so-called 'ASIA' (Autoimmune/inflammatory Syndrome Induced by Adjuvants) syndrome has been recently described, which is caused by the exposure to a component reproducing the effect of adjuvants. The aim of our study was to evaluate the effect of injection of complete Freund's adjuvant (CFA) in NZB/NZWF1 mice, a lupus-prone murine model. We injected 10 NZB/NZWF1 mice with CFA/PBS and 10 with PBS, three times, 3 weeks apart, and followed-up until natural death. CFA-injected mice developed both anti-double-stranded DNA and proteinuria earlier and at higher levels than the control group. Proteinuria-free survival rate and survival rate were significantly lower in CFA-treated mice than in the control mice (p = 0.002 and p = 0.001, respectively). Histological analyses showed a more severe glomerulonephritis in CFA-injected mice compared with the control mice. In addition, lymphoid hyperplasia in spleen and lungs, myocarditis, and vasculitis were observed in the former, but not in the latter group. In conclusion, the injection of CFA in NZB/NZWF1 mice accelerated autoimmune manifestations resembling 'ASIA' syndrome in humans. Lupus (2012) 21, 203-209.

引用

页码：203 / 209

页数：7

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