NMR-based plant metabolomics: where do we stand, where do we go?

被引:270
作者
Kim, Hye Kyong [1 ]
Choi, Young Hae [1 ]
Verpoorte, Robert [1 ]
机构
[1] Leiden Univ, Inst Biol, Sect Metabol, NL-2300 RA Leiden, Netherlands
关键词
MAGNETIC-RESONANCE-SPECTROSCOPY; IONIC LIQUIDS; H-1-NMR SPECTROSCOPY; QUALITY-CONTROL; IDENTIFICATION; CLASSIFICATION; EXTRACTS; METABONOMICS; METABOLITES; RESISTANCE;
D O I
10.1016/j.tibtech.2011.02.001
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
NMR-based metabolomics is an important tool for studying biological systems and has been applied in various organisms, including animals, plants and microbes. NMR is able to provide a 'holistic view' of the metabolites under certain conditions, and thus is advantageous for metabolomic studies. To maximize the use of the information obtained, it is also important to create a platform to measure, store and share data. Public databases for storing and sharing information are still lacking for NMR-based metabolomic analysis in plants. Such databases are urgently needed to make metabolic profiling a real omics technology. In addition, to understand metabolic processes in depth, single-cell analysis and the turnover of metabolites in pathways (fluxomics) should be measured.
引用
收藏
页码:267 / 275
页数:9
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