Disposable Biosensor for Measuring Sugars in Potatoes and Assessing Acrylamide Formation

We developed a disposable biosensor that combines the concept of first generation glucose biosensors (based on the glucose oxidase enzyme) with mediated detection of hydrogen peroxide (based on the horseradish peroxidase enzyme and ferrocene mediator). A specific ferrocene derivative, 1,1'-ferrocene diacetic acid (Fe(C(5)H(4)CH(2)COOH)(2)), was selected to avoid interference between the two enzymatic systems. The biosensor was able to measure low concentrations of glucose (0.01-1 mM), making it useful to determine the glucose content of potatoes and evaluate the risk of acrylamide formation. The biosensor showed excellent analytical properties in terms of stability, sensitivity, reproducibility, and accuracy. The biosensor was used to quantify glucose in different varieties of potatoes, and the results were validated by comparisons with those obtained by techniques commonly used in the potato processing industry.