Core-Shell HA-AuNPs@SiNPs Nanoprobe for Sensitive Fluorescence Hyaluronidase Detection and Cell Imaging

被引：35

作者：

Ge, Jia ^{[1
,2
,3
,4
]}

Cai, Ren ^{[2
,3
,4
]}

Yang, Lu ^{[2
,3
,4
]}

Zhang, Liangliang ^{[2
,3
,4
,7
]}

Jiang, Ying ^{[2
,3
,4
]}

Yang, Yu ^{[2
,3
,4
]}

Cui, Cheng ^{[2
,3
,4
]}

Wan, Shuo ^{[2
,3
,4
]}

Chu, Xia ^{[5
,6
]}

Tan, Weihong ^{[2
,3
,4
,5
,6
]}

机构：

[1] Zhengzhou Univ, Coll Chem & Mol Engn, 101 Kexue Rd, Zhengzhou 450001, Henan, Peoples R China

[2] Univ Florida, Ctr Res Bio Nano Interface, Dept Chem, Gainesville, FL 32611 USA

[3] Univ Florida, Dept Physiol & Funct Genom, Shands Canc Ctr, UF Genet Inst, Gainesville, FL 32611 USA

[4] Univ Florida, McKnight Brain Inst, Gainesville, FL 32611 USA

[5] Hunan Univ, State Key Lab Chemo Biosensing & Chemometr, 2 Lushan Rd, Changsha 410082, Hunan, Peoples R China

[6] Hunan Univ, Coll Chem & Chem Engn, 2 Lushan Rd, Changsha 410082, Hunan, Peoples R China

[7] Guangxi Normal Univ, State Key Lab Chem & Mol Engn Med Resources, Sch Chem & Pharmaceut Sci, 15 Yucai Rd, Guilin 541004, Peoples R China

来源：

ACS SUSTAINABLE CHEMISTRY & ENGINEERING | 2018年 / 6卷 / 12期

基金：

美国国家卫生研究院; 中国国家自然科学基金;

关键词：

Silicon nanoparticles; Fluorescence sensing; Hyaluronidase detection; Gold nanoparticles; Live-cell imaging; LABEL-FREE; GOLD NANOPARTICLES; ENZYMATIC-ACTIVITY; PLATE ASSAY; ACID; URINE; CYTOTOXICITY; SUBSTRATE; STRATEGY; HYAL1;

D O I：

10.1021/acssuschemeng.8b03684

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A highly sensitive and selective fluorescence strategy for hyaluronidase assay was developed by coupling silicon nanoparticles (SiNPs) as fluorescence indicators with hyaluronic-acid-functionalized gold nanoparticles (HA-AuNPs) as quenchers. With the formation of a core-shell HA-AuNPs@SiNPs nanoprobe by self-assembly, the fluorescence of SiNPs could be quenched by HA-AuNPs through fluorescence resonance energy transfer (FRET). The addition of hyaluronidase leads to cleavage of HA into small fragments, and SiNPs are released. As a result, an immediately fluorescent recovery appears when HAase is introduced. The proposed fluorescence method showed a linear response ranged for HAase from 0.01 to 10 U/mL, and a detection limit of 0.004 U/mL (S/N = 3) was obtained. Furthermore, it can be employed as an enzyme activatable nanoprobe for cell imaging applied in diagnostic and related biological studies.

引用

页码：16555 / 16562

页数：15

共 46 条

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