Biosynthesis of a lactate (LA)-based polyester with a 96 mol% LA fraction and its application to stereocomplex formation

被引：45

作者：

Shozui, Fumi ^{[1
]}

Matsumoto, Ken'ichiro ^{[1
]}

Motohashi, Ren ^{[1
]}

Sun, Jian ^{[1
]}

Satoh, Toshifumi ^{[1
]}

Kakuchi, Toyoji ^{[1
]}

Taguchi, Seiichi ^{[1
]}

机构：

[1] Hokkaido Univ, Grad Sch Engn, Div Biotechnol & Macromol Chem, Kita Ku, Sapporo, Hokkaido 0608628, Japan

来源：

POLYMER DEGRADATION AND STABILITY | 2011年 / 96卷 / 04期

关键词：

Polylactide; Biodegradable plastic; Microbial cell factory; PDLA; Biological PLA; 3-KETOACYL-ACP SYNTHASE III; ESCHERICHIA-COLI JM109; BETA-OXIDATION; POLYHYDROXYALKANOATE COPOLYMER; MICROBIAL-PRODUCTION; POLYMERIZING ENZYME; RALSTONIA-EUTROPHA; THERMAL-PROPERTIES; GENES; GLUCOSE;

D O I：

10.1016/j.polymdegradstab.2011.01.007

中图分类号：

O63 [高分子化学（高聚物）];

学科分类号：

070305 ; 080501 ; 081704 ;

摘要：

A poly(lactic acid) (PLA)-like terpolyester consisting of 96 mol% lactate (LA). I mol% 3-hydroxybutyrate and 3 mol% 3-hydroxyvalerate was produced in recombinant Escherichia coli LS5218 expressing LA-polymerizing enzyme (LPE). The strain was grown on glucose with a feeding of valerate as the monomer precursor. The glass transition and melting temperatures of the terpolyester were close to those of chemically synthesized poly(L-LA)s (PLLAs) having similar molecular weights. Additionally, a blend of the terpolyester, which was composed entirely of (R)-LA (D-LA) due to the strict enantiospecificity of LPE, with PLLA formed a stereocomplex with higher melting temperature (201.9 degrees C). These results indicate that the biological PLA-like polyester produced via this one-step microbial process has comparable thermal properties to chemically synthesized PLAs. (C) 2011 Elsevier Ltd. All rights reserved.

引用

页码：499 / 504

页数：6

共 32 条

[1] THERMAL PROPERTIES OF POLYLACTIDES Effect of molecular mass and nature of lactide isomer [J].