An α/β-HSQC-α/β experiment for spin-state selective editing of IS cross peaks

被引：66

作者：

Andersson, P

Annila, A

Otting, G ^{[1
]}

机构：

[1] Karolinska Inst, Dept Med Biochem & Biophys, S-17177 Stockholm, Sweden

[2] VTT Chem Technol, FIN-01044 Espoo, Finland

来源：

JOURNAL OF MAGNETIC RESONANCE | 1998年 / 133卷 / 02期

关键词：

one-bond coupling constants; Staphylococcus aureus gyrase B; TROSY; alpha/beta-HSQC-alpha/beta; spin-state selective editing;

D O I：

10.1006/jmre.1998.1492

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A generalized version of the TROSY experiment allows the spin-state selective editing of the four multiplet components of N-15-H-1 cross peaks of amide groups in proteins into four different subspectra, with no penalty in sensitivity. An improvement by root 2 in sensitivity results, if only two of the four multiplet components are selected. Use of the experiment for the measurement of 1J(HN) coupling constants is discussed. A water flip-back version of the experiment is demonstrated with a 45 kDa fragment of N-15/H-2 labeled Staphylococcus aureus gyrase B. (C) 1998 Academic Press.

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收藏

页码：364 / 367

页数：4

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