Self-folding micropatterned polymeric containers

被引:136
作者
Azam, Anum [1 ]
Laflin, Kate E. [1 ]
Jamal, Mustapha [1 ]
Fernandes, Rohan [1 ]
Gracias, David H. [1 ,2 ]
机构
[1] Johns Hopkins Univ, Dept Chem & Biomol Engn, Baltimore, MD 21218 USA
[2] Johns Hopkins Univ, Dept Chem, Baltimore, MD 21218 USA
关键词
Cell encapsulation therapy; Tissue engineering; Drug delivery; Microcontainers; Lithography; Bio-MEMS; POLYCAPROLACTONE; FABRICATION; DRIVEN; MICROCONTAINERS; SCAFFOLDS; DELIVERY; MOBILE;
D O I
10.1007/s10544-010-9470-x
中图分类号
R318 [生物医学工程];
学科分类号
0831 ;
摘要
We demonstrate self-folding of precisely patterned, optically transparent, all-polymeric containers and describe their utility in mammalian cell and microorganism encapsulation and culture. The polyhedral containers, with SU-8 faces and biodegradable polycaprolactone (PCL) hinges, spontaneously assembled on heating. Self-folding was driven by a minimization of surface area of the liquefying PCL hinges within lithographically patterned two-dimensional (2D) templates. The strategy allowed for the fabrication of containers with variable polyhedral shapes, sizes and precisely defined porosities in all three dimensions. We provide proof-of-concept for the use of these polymeric containers as encapsulants for beads, chemicals, mammalian cells and bacteria. We also compare accelerated hinge degradation rates in alkaline solutions of varying pH. These optically transparent containers resemble three-dimensional (3D) micro-Petri dishes and can be utilized to sustain, monitor and deliver living biological components.
引用
收藏
页码:51 / 58
页数:8
相关论文
共 36 条
  • [1] Mircofabrication technology for polycaprolactone, a biodegradable polymer
    Armani, DK
    Liu, C
    [J]. JOURNAL OF MICROMECHANICS AND MICROENGINEERING, 2000, 10 (01) : 80 - 84
  • [2] Three Dimensional Nanofabrication Using Surface Forces
    Cho, Jeong-Hyun
    Azam, Anum
    Gracias, David H.
    [J]. LANGMUIR, 2010, 26 (21) : 16534 - 16539
  • [3] Self-Assembly of Lithographically Patterned Nanoparticles
    Cho, Jeong-Hyun
    Gracias, David H.
    [J]. NANO LETTERS, 2009, 9 (12) : 4049 - 4052
  • [4] Precipitation casting of polycaprolactone for applications in tissue engineering and drug delivery
    Coombes, AGA
    Rizzi, SC
    Williamson, M
    Barralet, JE
    Downes, S
    Wallace, WA
    [J]. BIOMATERIALS, 2004, 25 (02) : 315 - 325
  • [5] Desai TA, 1998, BIOTECHNOL BIOENG, V57, P118, DOI 10.1002/(SICI)1097-0290(19980105)57:1<118::AID-BIT14>3.0.CO
  • [6] 2-G
  • [7] Directed assembly of cell-laden microgels for fabrication of 3D tissue constructs
    Du, Yanan
    Lo, Edward
    Ali, Shamsher
    Khademhosseini, Ali
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2008, 105 (28) : 9522 - 9527
  • [8] Micro-Masonry: Construction of 3D Structures by Microscale Self-Assembly
    Fernandez, Javier G.
    Khademhosseini, Ali
    [J]. ADVANCED MATERIALS, 2010, 22 (23) : 2538 - 2541
  • [9] Remote loading of doxorubicin into liposomes driven by a transmembrane phosphate gradient
    Fritze, Andreas
    Hens, Felicitas
    Kimpfler, Andrea
    Schubert, Rolf
    Peschka-Suess, Regine
    [J]. BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES, 2006, 1758 (10): : 1633 - 1640
  • [10] Self-assembled three dimensional radio frequency (RF) shielded containers for cell encapsulation
    Gimi, B
    Leong, T
    Gu, ZY
    Yang, M
    Artemov, D
    Bhujwalla, ZM
    Gracias, DH
    [J]. BIOMEDICAL MICRODEVICES, 2005, 7 (04) : 341 - 345