Solanum lycopersicum microRNA1916 targets multiple target genes and negatively regulates the immune response in tomato

被引：39

作者：

Chen, Lei ^{[1
]}

Meng, Jun ^{[2
]}

He, Xiao Li ^{[1
]}

Zhang, Min ^{[1
]}

Luan, Yu Shi ^{[1
]}

机构：

[1] Dalian Univ Technol, Sch Life Sci & Biotechnol, Dalian 116024, Peoples R China

[2] Dalian Univ Technol, Sch Comp Sci & Technol, Dalian 116024, Peoples R China

来源：

PLANT CELL AND ENVIRONMENT | 2019年 / 42卷 / 04期

基金：

中国国家自然科学基金;

关键词：

anthocyanins; Botrytis cinerea; miR1916; Phytophthora infestans; Solanum lycopersicum; strictosidine synthase; alpha-tomatine; PHYTOPHTHORA-INFESTANS; SEQUENCING REVEALS; ARTIFICIAL MICRORNAS; SMALL RNA; ARABIDOPSIS; PLANT; EXPRESSION; TRANSCRIPTION; BIOSYNTHESIS; METABOLISM;

D O I：

10.1111/pce.13468

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

MicroRNA1916 (miR1916) is one of the nonconserved miRNAs that respond to various stresses in plants, but little has been known at present about its mechanisms in biotic stresses. In this study, the expression of Solanum lycopersicum (sly)-miR1916 in tomato was found to be down-regulated after infection with Phytophthora infestans or Botrytis cinerea. Tomato plants that overexpressed sly-miR1916 displayed significant enhancement in susceptibility to P. infestans and B. cinerea infection, as well as increased tendency to produce reactive oxygen species. Silencing of sly-miR1916 by short tandem target mimic and artificial microRNA strategies caused the tomato plants to become more tolerant to adverse conditions. In addition, lower sly-miR1916 expression could up-regulate the expression of strictosidine synthase (STR-2), UDP-glycosyltransferases (UGTs), late blight resistance protein homolog R1B-16, disease resistance protein RPP13-like, and MYB transcription factor (MYB12), which ultimately resulted in the accumulation of alpha-tomatine and anthocyanins via STR-2, UGT, and MYB12. Furthermore, ectopic expression of sly-miR1916/STR-2 significantly changed the tolerance of tobacco to B. cinerea. Taken together, the results demonstrated that sly-miR1916 might regulate the expression of STR-2, UGT, and MYB12 in tomato plant, conferring sensitivity to biotic stress via modulating alpha-tomatine and anthocyanins.

引用

页码：1393 / 1407

页数：15

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