Strain Development and Medium Optimization for Fumaric Acid Production

被引：19

作者：

Kang, Seong Woo ^{[1
]}

Lee, Hawon ^{[2
]}

Kim, Daeheum ^{[2
]}

Lee, Dohoon ^{[3
]}

Kim, Sangyong ^{[3
]}

Chun, Gie-Taek ^{[4
]}

Lee, Jinwon ^{[5
]}

Kim, Seung Wook ^{[1
]}

Park, Chulhwan ^{[2
]}

机构：

[1] Korea Univ, Dept Chem & Biol Engn, Seoul 136701, South Korea

[2] Kwangwoon Univ, Dept Chem Engn, Seoul 139701, South Korea

[3] Korea Inst Ind Technol KITECH, Green Proc R&D Dept, Cheonan 330825, South Korea

[4] Kangwon Natl Univ, Sch Biosci & Biotechnol, Chunchon 200701, South Korea

[5] Sogang Univ, Dept Chem & Biomol Engn, Seoul 121742, South Korea

来源：

BIOTECHNOLOGY AND BIOPROCESS ENGINEERING | 2010年 / 15卷 / 05期

关键词：

Rhizopus oryzae; fumaric acid; response surface methodology (RSM); mutagenesis; optimization; ROTARY BIOFILM CONTACTOR; RHIZOPUS-ARRHIZUS; FERMENTATION; METHODOLOGY; ORYZAE;

D O I：

10.1007/s12257-010-0081-4

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Rhizopus gate RUR709 mutant was isolated based on halo size from selection medium via mutagenesis with UV and gamma-rays, and the production of fumaric acid in the submerged fermentation was assessed. The maximum concentration of fumaric acid was obtained using 0.5% corn steep liquor (CSL) as the nitrogen source. Organic nitrogen sources were shown to be more effective in fumaric acid production than inorganic nitrogen sources. Using optimum medium obtained by response surface methodology (RSM), the maximum concentration of fumaric acid achieved in flask culture was 26.2 g/L, which is fairly close to the 27.4 g/L predicted by the model. The highest concentration of fumaric acid in the stirred-tank reactor generated by the R. oryzae RUR709 mutant was 32.1 g/L and yield (0.45 g/g) and productivity (0.32 g/L/h) were highest at 4 days.

引用

页码：761 / 769

页数：9

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