The inhibitory effect Polygonum Cillinerve polysaccharide on transmissible gastroenteritis virus of swine

被引：16

作者：

Pan, Xingxue ^{[1
]}

Zhou, Yu ^{[1
]}

Duan, Xueqin ^{[1
]}

Cui, Jing ^{[1
]}

Liu, Jia ^{[1
]}

Song, Xiaoping ^{[1
]}

Ma, Wuren ^{[1
]}

Zhang, Weimin ^{[1
]}

Liu, Yingqiu ^{[1
]}

Fan, Yunpeng ^{[1
]}

机构：

[1] Northwest A&F Univ, Coll Vet Med, Yangling 712100, Shaanxi, Peoples R China

来源：

RESEARCH IN VETERINARY SCIENCE | 2021年 / 140卷

关键词：

Transmissible gastroenteritis virus of swine; Polygonum Cillinerve polysaccharide; Cell apoptosis; A VIRUS; INFECTION; APOPTOSIS; BAX; ROS;

D O I：

10.1016/j.rvsc.2021.08.005

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

Transmissible gastroenteritis virus of swine (TGEV) is one kind of the main pathogens causing viral diarrhea in pig. In this study, the inhibitory effect of Polygonum Cillinerve polysaccharide (PCP) on TGEV was studied. Firstly, MTT method was used to measure the cell viability of PCP. Then Hoechst 33258 fluorescence staining, Annexin V-FITC/PI fluorescence staining, real-time PCR and western blot were used to explore the effect of PCP on inhibiting TGEV. The results showed that PCP could significantly reduce the apoptosis rate induced by TGEV, reduce the expression of ROS, reduce TGEV replication, increase the expression levels of Bcl-2 and Bax genes, increase the expression of Bcl-2 protein, decreased the expression of Cyto c protein, and reduce the amount of cleaved caspase 3. Therefore, PCP had the better inhibitory effect on TGEV, which provided a certain theoretical basis for the prevention and treatment of TGEV.

引用

页码：47 / 55

页数：9

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