cDNA Cloning and Expression of Cyclophilin A (LvCypA) in White Leg Shrimp, Litopenaeus vannamei

被引：5
作者：
Muhammad, Faiz [1 ,2 ]
Zhi-Feng, Zhang [1 ]
Ming-Yu, Shao [1 ]
Shafi, Muhammad [3 ]
机构：
[1] Ocean Univ China, Minist Educ, Key Lab Marine Genet & Breeding, Qingdao 266003, Peoples R China
[2] Univ Karachi, Ctr Excellence Marine Biol, Karachi, Pakistan
[3] Lasbela Univ Agr Water & Marine Sci, Uthal, Pakistan
来源：
PAKISTAN JOURNAL OF ZOOLOGY | 2017年 / 49卷 / 03期
关键词：
Cloning; Cyclophilin A gene expression; Cyclophilin A; Litopenaeus vannamei; CIS-TRANS-ISOMERASE; BINDING-PROTEIN; CYCLOSPORINE-A; IMMUNOPHILINS; MECHANISM; CD147;
D O I：
10.17582/journal.pjz/2017.49.3.935.941
中图分类号：
Q95 [动物学];
学科分类号：
071002 ;
摘要：
In present investigation the Cyclophilin A (CypA) of white leg shrimp (Litopenaeus vannamei) has been cloned by rapid amplification of cDNA (RACE) and anchored PCR method. The full length of the (LvCypA) had 855 bp, containing 495 bp of open reading frame (ORF), encoding 164 amino acids with an estimated molecular mass of 17.6 kDa. The LvCypA has four 13-strands. Tissue distribution of the LvCypA after real time analysis revealed that expression is in order of muscle, gill, lymphoid organ and hepatopancrease.
引用
收藏
页码：935 / 941
页数：7
相关论文
共 37 条
  • [1] AUSTIN B, 1988, MARINE MICROBIOLOGY, P166
  • [2] AIF and cyclophilin A cooperate in apoptosis-associated chromatinolysis
    Candé, C
    Vahsen, N
    Kouranti, I
    Schmitt, E
    Daugas, E
    Spahr, C
    Luban, J
    Kroemer, RT
    Giordanetto, F
    Garrido, C
    Penninger, JM
    Kroemer, G
    [J]. ONCOGENE, 2004, 23 (08) : 1514 - 1521
  • [3] Molecular cloning and characterization of two isoforms of cyclophilin A gene from Venerupis philippinarum
    Chen, Leilei
    Mu, Changkao
    Zhao, Jianmin
    Wang, Chunlin
    [J]. FISH & SHELLFISH IMMUNOLOGY, 2011, 31 (06) : 1218 - 1223
  • [4] Enzyme dynamics during catalysis
    Eisenmesser, EZ
    Bosco, DA
    Akke, M
    Kern, D
    [J]. SCIENCE, 2002, 295 (5559) : 1520 - 1523
  • [5] CYCLOPHILIN AND PEPTIDYL-PROLYL CIS-TRANS ISOMERASE ARE PROBABLY IDENTICAL PROTEINS
    FISCHER, G
    WITTMANNLIEBOLD, B
    LANG, K
    KIEFHABER, T
    SCHMID, FX
    [J]. NATURE, 1989, 337 (6206) : 476 - 478
  • [6] IMMUNOPHILINS IN PROTEIN-FOLDING AND IMMUNOSUPPRESSION
    FRUMAN, DA
    BURAKOFF, SJ
    BIERER, BE
    [J]. FASEB JOURNAL, 1994, 8 (06) : 391 - 400
  • [7] Variations of sequences and amino acid compositions of proteins that sustain their biological functions: An analysis of the cyclophilin family of proteins
    Galat, A
    [J]. ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1999, 371 (02) : 149 - 162
  • [8] Gunalan Balakrishnan Gunalan Balakrishnan, 2011, Advances in Applied Science Research, V2, P107
  • [9] CYCLOPHILIN - A SPECIFIC CYTOSOLIC BINDING-PROTEIN FOR CYCLOSPORIN-A
    HANDSCHUMACHER, RE
    HARDING, MW
    RICE, J
    DRUGGE, RJ
    [J]. SCIENCE, 1984, 226 (4674) : 544 - 547
  • [10] Structural insights into the catalytic mechanism of cyclophilin A
    Bruce R Howard
    Felix F Vajdos
    Su Li
    Wesley I Sundquist
    Christopher P Hill
    [J]. Nature Structural & Molecular Biology, 2003, 10 (6) : 475 - 481
1234