Phase image correlation spectroscopy for detecting microfluidic dynamics

被引：5

作者：

Yu, Lan ^{[1
]}

Wang, Yu ^{[1
]}

Wang, Yang ^{[1
]}

Zhuo, Kequn ^{[1
]}

Ma, Ying ^{[1
]}

Liu, Min ^{[1
]}

Zheng, Juanjuan ^{[1
,2
]}

Li, Jianlang ^{[1
]}

Li, Junhua ^{[3
]}

Gao, Peng ^{[1
]}

机构：

[1] Xidian Univ, Sch Phys & Optoelect Engn, Xian 710071, Peoples R China

[2] Chinese Acad Sci, Xian Inst Opt & Precis Mech, State Key Lab Transient Opt & Photon, Xian 710119, Peoples R China

[3] Nanchang Hangkong Univ, Key Lab Jiangxi Prov Image Proc & Pattern Recogni, Nanchang 330063, Jiangxi, Peoples R China

来源：

APPLIED OPTICS | 2022年 / 61卷 / 20期

基金：

中国国家自然科学基金;

关键词：

FLUORESCENCE CORRELATION SPECTROSCOPY; SINGLE-PARTICLE TRACKING; VELOCIMETRY; MICROSCOPY; DIFFUSION; FLOW;

D O I：

10.1364/AO.458026

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

It is essential to quantify the physical properties and the dynamics of flowing particles in many fields, especially in microfluidic-related applications. We propose phase image correlation spectroscopy (PICS) as a versatile tool to quantify the concentration, hydro-diameter, and flow velocity of unlabeled particles by correlating the pixels of the phase images taken on flowing particles in a microfluidic device. Compared with conventional image correlation spectroscopy, PICS is minimally invasive, relatively simple, and more efficient, since it utilizes the intrinsic phase of the particles to provide a contrast instead of fluorescent labeling. We demonstrate the feasibility of PICS by measuring flowing polymethylmethacrylate (PMMA) microspheres and yeast in a microfluidic device. We can envisage that PICS will become an essential inspection tool in biomedicine and industry. (C) 2022 Optica Publishing Group

引用

页码：5944 / 5950

页数：7

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