Removal of contaminating DNA from commercial nucleic acid extraction kit reagents

被引：50
作者：
Mohammadi, T
Reesink, HW
Vandenbroucke-Grauls, CMJE
Savelkoul, PHM
机构：
[1] VU Univ, Med Ctr, Dept Med Microbiol & Infect Control, NL-1007 MB Amsterdam, Netherlands
[2] Sanquin Blood Bank NW Reg, Amsterdam, Netherlands
来源：
JOURNAL OF MICROBIOLOGICAL METHODS | 2005年 / 61卷 / 02期
关键词：
contamination; real time PCR; universal PCR; platelet concentrates;
D O I：
10.1016/j.mimet.2004.11.018
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Due to contamination of DNA extraction reagents, false-positive results can occur when applying broad-range real-time PCR based on bacterial 16S rDNA. Filtration of the nucleic acid extraction kit reagents with GenElute Maxiprep binding columns was effective in removing this reagent-derived contaminating DNA while the sensitivity of the assay was maintained. (C) 2004 Elsevier B.V. All rights reserved.
引用
收藏
页码：285 / 288
页数：4
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