Scanning electrochemical microscopy of single human urinary bladder cells using reactive oxygen species as probe of inflammatory response

被引：30

作者：

Zhao, Xiaocui ^{[1
]}

Lam, Simon ^{[2
,3
]}

Jass, Jana ^{[2
,3
,4
]}

Ding, Zhifeng ^{[1
]}

机构：

[1] Univ Western Ontario, Dept Chem, London, ON N6A 5B7, Canada

[2] Univ Western Ontario, Lawson Hlth Res Inst, London, ON N6A 5B7, Canada

[3] Univ Western Ontario, Dept Microbiol & Immunol, London, ON N6A 5B7, Canada

[4] Univ Orebro, Orebro Life Sci Ctr, Sch Sci & Technol, S-70182 Orebro, Sweden

来源：

ELECTROCHEMISTRY COMMUNICATIONS | 2010年 / 12卷 / 06期

基金：

加拿大自然科学与工程研究理事会;

关键词：

Scanning electrochemical microscopy; Reactive oxygen species; Ultramicroelectrode; Inflammatory response; Live cells; Toll-like receptors; OXIDATIVE STRESS; LIVING CELLS; DIFFERENTIATION; TRANSCRIPTION; RECEPTORS; DIFFUSION;

D O I：

10.1016/j.elecom.2010.03.030

中图分类号：

O646 [电化学、电解、磁化学];

学科分类号：

081704 ;

摘要：

Scanning electrochemical microscopy of single human bladder (T24) and kidney (A498) epithelial cells after stimulation with heat-killed uropathogenic Escherichia coli GR-12 was investigated for the first time. 124 cells treated with the hk-UPEC in time-lapsed images showed prolonged release of reactive oxygen species (ROS) due to toll-like receptors (TLR4), while A498 cells without TLR4 did not show the ROS increase after the dosage of the same stimulant. This indicates discrete pathways of triggering the inflammatory. It also reveals that ROS can be used as a probe to detect inflammatory response to gram-negative bacteria. (C) 2010 Elsevier B.V. All rights reserved.

引用

页码：773 / 776

页数：4

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