Characterization and optimization of production of bacterial cellulose from strain CGMCC 17276 based on whole-genome analysis

被引：68

作者：

Lu, Tingfen ^{[1
,2
]}

Gao, Hongliang ^{[1
,2
]}

Liao, Bowen ^{[1
,2
]}

Wu, Jiajing ^{[1
,2
]}

Zhang, Wei ^{[1
,2
]}

Huang, Jie ^{[1
,2
]}

Liu, Mingyao ^{[1
,2
]}

Huang, Jing ^{[1
,2
]}

Chang, Zhongyi ^{[1
,2
]}

Jin, Mingfei ^{[1
,2
]}

Yi, Zhengfang ^{[1
,2
]}

Jiang, Deming ^{[1
,2
]}

机构：

[1] East China Normal Univ, East China Normal Univ & Shanghai Fengxian Dist C, Shanghai Key Lab Regulatory Biol, Inst Biomed Sci, Shanghai 200241, Peoples R China

[2] East China Normal Univ, Sch Life Sci, Shanghai 200241, Peoples R China

来源：

CARBOHYDRATE POLYMERS | 2020年 / 232卷

基金：

中国国家自然科学基金;

关键词：

Komagataeibacter; Bacterial cellulose; Whole-genome analysis; Optimization of fermentation medium; Waste glycerol; DIFFERENT CARBON-SOURCES; ACETOBACTER SP V6; GLUCONACETOBACTER-XYLINUS; AGITATED CULTURE; SP-NOV; HANSENII; SEQUENCE; BIOSYNTHESIS; SYNTHASE; GENES;

D O I：

10.1016/j.carbpol.2019.115788

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

Bacterial cellulose (BC) has received considerable attention as an environment-friendly, biodegradable nano-material. In this study, the strain Komagataeibacter sp. nov. CGMCC 17276, which showed rapid cell growth and high BC-production ability, was isolated and classified into a novel species in the Komagataeibacter genus. Four BC synthase operons were annotated using whole-genome analysis, partially explaining the high BC yield of strain CGMCC 17276. Operons bcs. and bcs. showed high transcriptional levels under static and agitated culture conditions, indicating their importance in BC synthesis. Of the eight suitable carbon sources identified by whole-genome analysis, the highest BC production was achieved using glycerol as a single carbon source. Finally, waste glycerol was successfully used as an eco-friendly and sustainable strategy for BC production. This study provides valuable insights into the mechanism of BC synthesis, genetic structure of BC-producing strains, and industrialization of BC production using an eco-friendly and low-cost strategy.

引用

页数：14

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