Assessment of genetic polymorphism in hop (Humulus lupulus L.) cultivars by ISSR-PCR analysis

被引:9
作者
Danilova, TV [1 ]
Danilov, SS
Karlov, GI
机构
[1] KA Timiryazev Agr Acad, Moscow 127550, Russia
[2] Res & Technol Inst Hop Prod, Tsivilsk 429990, Chuvashia, Russia
关键词
D O I
10.1023/B:RUGE.0000004140.26775.db
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
Genetic diversity among 26 Russian and European cultivars of the common hop (Humulus lupulus L.) was studied using the ISSR - PCR technique. Twenty-one primers used provided amplification of 183 DNA fragments, 106 of which (57.9%) were found to be polymorphic. The ISSR markers, specific for some cultivars were revealed. Based on the genetic distances, cluster analysis was performed and a dendrogram was constructed, on which most of the hop cultivars formed two clusters according to their origin. Advantages of the ISSR - PCR analysis in breeding studies for classification and identification of common hop cultivars are discussed.
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页码:1252 / 1257
页数:6
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