Umbilical cord transforming growth factor-beta 3: Isolation, comparison with recombinant TGF-beta 3 and cellular localization

被引：11

作者：

Stewart, AA

Haley, JD

Qu, GY

Stam, K

Fenyo, D

Chait, BT

Marshak, DR

Ng, AYK

Marley, G

Iwata, KK

机构：

[1] ONCOGENE SCI,DIV PHARMACEUT,UNIONDALE,NY 11553

[2] ROCKEFELLER UNIV,MASS SPECTROMETRY UNIT,NEW YORK,NY 10021

[3] WM KECK STRUCT BIOL LAB,COLD SPRING HARBOR,NY 11724

[4] BECKMAN NEUROSCI CTR,COLD SPRING HARBOR,NY 11724

[5] UROCOR INC,OKLAHOMA CITY,OK 73112

来源：

GROWTH FACTORS | 1996年 / 13卷 / 1-2期

关键词：

transforming growth factor beta-3; TGF-beta; 3; growth factor purification; umbilical cord; mass spectrometry; TGI;

D O I：

10.3109/08977199609034569

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The transforming growth factor beta (TGF-beta) family of growth modulators play critical roles in tissue development and maintenance. Recent data suggest that individual TGF-beta isoforms (TGF-beta 1, -beta 2 and -beta 3) have overlapping yet distinct biological actions and target cell specificities, both in developing and adult tissues. The TGF-beta 3 isoform was purified to homogeneity from both natural and recombinant sources and characterized by laser desorption mass spectrometry, by protein sequencing, by amino acid analysis and by biological activity. TGF-beta 3 was the major TGF-beta isoform in umbilical cord (230 ng/g), and was physically and biologically indistinguishable from recombinant TGF-beta 3 and from the tumor growth inhibitory (TGI) protein found in umbilical cord. Immunohistochemistry using antipeptide TGF-beta 3 specific antibody showed TGF-beta 3 localization in perivascular smooth muscle.

引用

页码：87 / &

页数：13

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