Transplantation of cultured human corneal endothelial cells

被引:36
作者
Amano, S [1 ]
机构
[1] Univ Tokyo, Sch Med, Dept Ophthalmol, Bunkyo Ku, Tokyo 1138655, Japan
关键词
cell culture; cell senescence; corneal endothelial cell; porcine stroma; reconstructed cornea;
D O I
10.1097/00003226-200310001-00010
中图分类号
R77 [眼科学];
学科分类号
100212 ;
摘要
Purpose: To investigate the feasibility of corneal reconstruction utilizing cultured human corneal endothelial cells (HCECs). Methods: We cultivated HCECs using culture dishes pre-coated with bovine corneal. endothelial extracellular matrix. The effect of donor age on HCECs was investigated. We reconstructed corneas using cultured HCECs and human corneal stroma, then examined their functioning. The possibility of porcine corneal stroma as a carrier of cultured HCECs was investigated. Results: The older the donor, the more frequently large senescent cells appeared in the passaged HCECs. The density of HCECs on the reconstructed cornea reached 2500 cells/mm(2). The potential difference in the reconstructed and normal corneas was 0.30 mV and 0.40 mV, respectively; this indicates that the pump function of the reconstructed corneas is 75% of that of normal corneas. Porcine corneal stroma expressing little xenosugar antigen alpha-gal epitope induced no superacute rejection but mild cellular rejection when transplanted into corneas of animals possessing natural antibody to a-gal epitope. Conclusions: To reconstruct corneas that are the same as, or superior to, normal corneas, innovation is necessary in the methods used for culturing and seeding HCECs. Porcine corneal stroma is promising as a carrier of HCECs instead of human corneal stroma, the supply of which is limited. The validity of porcine corneal stroma, acellularized to prevent retrovirus infection, should be evaluated.
引用
收藏
页码:S66 / S74
页数:9
相关论文
共 35 条
[1]  
Amano Shiro, 2002, Nippon Ganka Gakkai Zasshi, V106, P805
[2]  
Blake DA, 1997, INVEST OPHTH VIS SCI, V38, P1119
[3]   12-YEAR COMPARISON OF A BJORK-SHILEY MECHANICAL HEART-VALVE WITH PORCINE BIOPROSTHESES [J].
BLOOMFIELD, P ;
WHEATLEY, DJ ;
PRESCOTT, RJ ;
MILLER, HC .
NEW ENGLAND JOURNAL OF MEDICINE, 1991, 324 (09) :573-579
[4]  
Böhnke M, 1999, CORNEA, V18, P207
[5]   Transplantation of adult human corneal endothelium ex vivo - A morphologic study [J].
Chen, KH ;
Azar, D ;
Joyce, NC .
CORNEA, 2001, 20 (07) :731-737
[6]   5 TO 8-YEAR FOLLOW-UP OF PATIENTS UNDERGOING PORCINE HEART-VALVE REPLACEMENT [J].
COHN, LH ;
MUDGE, GH ;
PRATTER, F ;
COLLINS, JJ .
NEW ENGLAND JOURNAL OF MEDICINE, 1981, 304 (05) :258-262
[7]   15-YEAR EXPERIENCE WITH 1678 HANCOCK PORCINE BIOPROSTHETIC HEART-VALVE REPLACEMENTS [J].
COHN, LH ;
COLLINS, JJ ;
DISESA, VJ ;
COUPER, GS ;
PEIGH, PS ;
KOWALKER, W ;
ALLRED, E .
ANNALS OF SURGERY, 1989, 210 (04) :435-443
[8]  
Egan CA, 1998, INVEST OPHTH VIS SCI, V39, P648
[9]  
ENGELMANN K, 1989, IN VITRO CELL DEV B, V25, P1065
[10]   Transplantation of adult human or porcine corneal endothelial cells onto human recipients in vitro.: Part I:: Cell culturing and transplantation procedure [J].
Engelmann, K ;
Drexler, D ;
Böhnke, M .
CORNEA, 1999, 18 (02) :199-206