Enzyme biosensor based on NAD-sensitive quantum dots

被引：38
作者：
Ren, Xiangling [1 ,2 ]
Yang, Liuqing [1 ,2 ,3 ]
Tang, Fangqiong [1 ,2 ]
Yan, Chuanmiao [1 ,2 ,3 ]
Ren, Jun [1 ,2 ]
机构：
[1] Chinese Acad Sci, Tech Inst Phys & Chem, Lab Controllable Preparat & Applicat Nanomat, Beijing 100190, Peoples R China
[2] Chinese Acad Sci, Grad Univ, Beijing 100190, Peoples R China
[3] Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
来源：
BIOSENSORS & BIOELECTRONICS | 2010年 / 26卷 / 01期
基金：
中国国家自然科学基金;
关键词：
Quantum dots; Fluorescence; Biosensors; Lactate dehydrogenase; Nicotinamide adenine dinucleotide; RESONANCE ENERGY-TRANSFER; LACTATE-DEHYDROGENASE; CANCER CELLS; HYBRIDIZATION; GLUCOSE; CDTE; NANOCRYSTALS; DONORS; ACID; PH;
D O I：
10.1016/j.bios.2010.05.014
中图分类号：
Q6 [生物物理学];
学科分类号：
071011 ;
摘要：
A novel quantum dots (QDs) based biosensor has been developed to detect the activity of lactate dehydrogenase (LDH) by the change of fluorescence intensities of the QDs In this system, the fluorescence intensities of the QDs are quenched by nicotinamide adenine cinucleotide (NAD. the coenzyme of LDH) first and then intensified with increasing amounts of the LDH because of the consumption of the NAD in the biocatalyzed reaction A linear calibration plot of the activity of LDH is obtained in the wide amounts range from 150 to 1500 U/L and the detection limit is 75 U/L Furthermore, the effect of several possible interfering substances, such as uric acid (UA), lactose, and glucose on the QDs-based biosensor is also investigated. This enzyme biosensor is of considerable interest due to its promise for simple procedure and the established method has great potential in detection of the physiological level of some biomolecules in clinical diagnostics of various diseases. (C) 2010 Elsevier B V. All rights reserved
引用
收藏
页码：271 / 274
页数：4
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