Micellar electrokinetic capillary chromatography with non-ionic surfactant tween 20

Three polyphenols, scopoletin, rutin and esculetin, could be successfully separated by using micellar electrokinetic capillary chromatographic (MEKC) method with non-ionic surfactant Tween 20 could successfully separate 3 polyphenols, scopoletin, rutin and esculetin. The running buffer of pH 9. 4, 60 mmol/L sodium tetraborate containing 50 mmol/L Tween 20 was used in the polyphenol separation. The equilibrium constant K of Tween 20-rutin and Tween 20 esculetin system were calculated to be 4. 53 X 10(5) and 2. 23 X 10(5) (L/mol) 2 respectively, by a spectrophotometric method. And both the binding numbers of them was 2. This shows that a satisfied separation of the polyphenols was achieved by the difference of stableness of cooperation betweenTween 20-rutin and Tween 20-esculetin and the different mobility of 3 of them.