Effect of fibroblast growth factor 9 on the osteogenic differentiation of bone marrow stromal stem cells and dental pulp stem cells

被引：23

作者：

Lu, Jingting ^{[1
]}

Dai, Jiewen ^{[1
]}

Wang, Xudong ^{[1
]}

Zhang, Maolin ^{[1
]}

Zhang, Peng ^{[1
]}

Sun, Hao ^{[1
]}

Zhang, Xiuli ^{[2
]}

Yu, Hongbo ^{[1
]}

Zhang, Wenbin ^{[1
]}

Zhang, Lei ^{[1
]}

Jiang, Xinquan ^{[2
]}

Shen, Steve Guofang ^{[1
]}

机构：

[1] Shanghai Jiao Tong Univ, Shanghai Peoples Hosp 9, Coll Stomatol, Dept Oral & Craniomaxillofacial Sci,Sch Med, Shanghai 200011, Peoples R China

[2] Shanghai Jiao Tong Univ, Shanghai Peoples Hosp 9, Shanghai Res Inst Stomatol, Oral Bioengn Lab,Shanghai Key Lab Stomatol,Sch Me, Shanghai 200011, Peoples R China

来源：

MOLECULAR MEDICINE REPORTS | 2015年 / 11卷 / 03期

关键词：

fibroblast growth factor 9; osteogenic differentiation; bone marrow stromal stem cells; dental pulp stem cells; CHONDROCYTE DIFFERENTIATION; FGF9; ANGIOGENESIS; OSSIFICATION; EXPRESSION; CARTILAGE; MICE;

D O I：

10.3892/mmr.2014.2998

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

The role of fibroblast growth factor 9 (FGF9) in bone formation may depend on gene dosage, developmental stage, cell type or interactions with other cytokines. In the present study bone marrow stromal stem cells (BMSCs) and dental pulp stem cells (DPSCs) were cultured and osteogenically induced in vitro, treated with exogenous FGF9 at varying concentrations. Alkaline phosphatase staining, alizarin red S staining, reverse transcription quantitative polymerase chain reaction and western blot analyses were performed in order to investigate the gene expression levels of osteogenic markers. The results of the present study demonstrated that FGF9 enhanced the phosphorylation of extracellular signal-regulated kinase 1/2 (ERK1/2) during osteogenic induction in BMSCs and DPSCs, which are derived from different tissues. FGF9 also inhibited the osteogenic differentiation of BMSCs and DPSCs through the activation of ERK1/2. These findings suggested that FGF9 may be an inhibitor of osteogenesis in mesenchymal stem cells in vitro and its application in,vivo requires investigation in the future.

引用

页码：1661 / 1668

页数：8

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