Nanopatterning the chemospecific immobilization of cowpea mosaic virus capsid

被引：124
作者：
Smith, JC
Lee, KB
Wang, Q
Finn, MG
Johnson, JE
Mrksich, M
Mirkin, CA
机构：
[1] Univ Chicago, Dept Chem, Chicago, IL 60637 USA
[2] Univ Chicago, Inst Biophys Dynam, Chicago, IL 60637 USA
[3] Northwestern Univ, Dept Chem, Evanston, IL 60208 USA
[4] Northwestern Univ, Inst Nanotechnol, Evanston, IL 60208 USA
[5] Scripps Res Inst, Dept Chem, La Jolla, CA 92037 USA
[6] Scripps Res Inst, Dept Mol Biol, La Jolla, CA 92037 USA
来源：
NANO LETTERS | 2003年 / 3卷 / 07期
关键词：
SELF-ASSEMBLED MONOLAYERS; DIP-PEN NANOLITHOGRAPHY; SUPRAMOLECULAR BUILDING-BLOCKS; PLASMON RESONANCE SPECTROSCOPY; SELECTIVE IMMOBILIZATION; PROTEIN-PATTERNS; GOLD; FABRICATION; MICROARRAYS; ADSORPTION;
D O I：
10.1021/nl025956h
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
This paper presents a flexible approach for using Dip Pen Nanolithography (DPN) to nanopattern mixed monolayers for the selective immobilization of bioassemblies. DPN was used with a binary ink-consisting of a symmetric 11-mercaptoundecyl-penta(ethylene glycol) disulfide and a mixed disulfide substituted with one malelmide group-to pattern nanoscale features that present functional groups for the chemospecific immobilization of cysteine-labeled biomolecules. This strategy was applied to the chemospecific immobilization of cysteine mutant cowpea mosaic virus capsid particles (cys-VCPs). The combination of DPN for defining nanopatterns and surface chemistries for controlling the immobilization of ligands will be broadly useful in basic and applied biology.
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收藏
页码：883 / 886
页数：4
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