Ascorbic acid protects the toxic effects of aflatoxin B1 on yak oocyte maturation

被引：3
作者：
Li, Qin [1 ]
Zhao, Tian [1 ]
He, Honghong [1 ]
Robert, Niayale [2 ]
Ding, Tianyi [1 ]
Hu, Xuequan [1 ]
Zhang, Tongxiang [1 ]
Pan, Yangyang [1 ]
Cui, Yan [2 ]
Yu, Sijiu [1 ]
机构：
[1] Gansu Agr Univ, Coll Vet Med, Gansu Prov Livestock Embryo Engn Res Ctr, Lanzhou, Gansu, Peoples R China
[2] Gansu Agr Univ, Coll Vet Med, Lab Anim Anat & Tissue Embryol, Lanzhou, Peoples R China
来源：
ANIMAL SCIENCE JOURNAL | 2022年 / 93卷 / 01期
关键词：
aflatoxin B-1; ascorbic acid; DNA methylation; oocytes; yak; VITAMIN-C; DEVELOPMENTAL COMPETENCE; IN-VITRO; DNA METHYLATION; ZONA-PELLUCIDA; FERTILIZATION; MITOCHONDRIA; HEALTH; GROWTH; EXPRESSION;
D O I：
10.1111/asj.13702
中图分类号：
S8 [畜牧、 动物医学、狩猎、蚕、蜂];
学科分类号：
0905 ;
摘要：
High-quality oocytes are a prerequisite for successful fertilization. Mammals feeding on aflatoxin-contaminated feed can cause reproductive toxicity, including follicular atresia, poor oocyte development and maturation, and aberrant epigenetic modifications of oocytes. In addition, the important role of ascorbic acid (AA) in reproductive biology has been confirmed, and AA is widely used as an antioxidant in cell culture. However, the toxic effects of aflatoxin B-1 (AFB(1)) on yak oocytes and whether AA has protective effects remain unknown. In this study, we found that exposure to AFB(1) impedes meiotic maturation of oocytes, promotes apoptosis by triggering high levels of reactive oxygen species (ROS), and disrupts mitochondrial distribution and actin integrity, resulting in a decrease in the fertilization ability and parthenogenetic development ability of oocytes. In addition, these injuries changed the DNA methylation transferase transcription level of mature oocytes. After adding 50 mu g/ml AA, the indices recovered to levels close to those of the control group. The results showed that AA could protect yak oocytes from the toxic effects of AFB(1) and improve the quality of oocytes.
引用
收藏
页数：11
相关论文
共 70 条
[1]   RETRACTED: Antioxidant nutrients and mycotoxins (Retracted Article) [J].
Atroshi, F ;
Rizzo, A ;
Westermarck, T ;
Ali-Vehmas, T .
TOXICOLOGY, 2002, 180 (02) :151-167
[2]   Molecular architecture of the human sperm IZUMO1 and egg JUNO fertilization complex [J].
Aydin, Halil ;
Sultana, Azmiri ;
Li, Sheng ;
Thavalingam, Annoj ;
Lee, Jeffrey E. .
NATURE, 2016, 534 (7608) :562-+
[3]   Aflatoxin Biosynthesis and Genetic Regulation: A Review [J].
Caceres, Isaura ;
Al Khoury, Anthony ;
El Khoury, Rhoda ;
Lorber, Sophie ;
Oswald, Isabelle ;
El Khoury, Andre ;
Atoui, Ali ;
Puel, Olivier ;
Bailly, Jean-Denis .
TOXINS, 2020, 12 (03)
[4]   The epigenetic role of vitamin C in health and disease [J].
Camarena, Vladimir ;
Wang, Gaofeng .
CELLULAR AND MOLECULAR LIFE SCIENCES, 2016, 73 (08) :1645-1658
[5]   Improving the Development of Early Bovine Somatic-Cell Nuclear Transfer Embryos by Treating Adult Donor Cells With Vitamin C [J].
Chen, Huanhuan ;
Zhang, Lei ;
Guo, Zekun ;
Wang, Yongsheng ;
He, Rongjun ;
Qin, Yumin ;
Quan, Fusheng ;
Zhang, Yong .
MOLECULAR REPRODUCTION AND DEVELOPMENT, 2015, 82 (11) :867-879
[6]   2,4-DCP compromises the fertilization capacity of mouse oocytes [J].
Dai, Xiaoxin ;
Qiu, Lijun ;
Rashida, Churi ;
Xu, Chunying ;
Mu, Yi ;
Gao, Yang ;
Chu, Zhangjie ;
Zhao, Bo .
JOURNAL OF CELLULAR PHYSIOLOGY, 2021, 236 (11) :7605-7611
[7]   Aflatoxin B1-induced epigenetic alterations: An overview [J].
Dai, Yaqi ;
Huang, Kunlun ;
Zhang, Boyang ;
Zhu, Liye ;
Xu, Wentao .
FOOD AND CHEMICAL TOXICOLOGY, 2017, 109 :683-689
[8]   Metabolism of aflatoxins: key enzymes and interindividual as well as interspecies differences [J].
Dohnal, Vlastimil ;
Wu, Qinghua ;
Kuca, Kamil .
ARCHIVES OF TOXICOLOGY, 2014, 88 (09) :1635-1644
[9]   Age related changes in mitochondrial function and new approaches to study redox regulation in mammalian oocytes in response to age or maturation conditions [J].
Eichenlaub-Ritter, U. ;
Wieczorek, M. ;
Lueke, S. ;
Seidel, T. .
MITOCHONDRION, 2011, 11 (05) :783-796
[10]   BIOLOGY AND STRUCTURE OF THE ZONA-PELLUCIDA - A TARGET FOR IMMUNOCONTRACEPTION [J].
EPIFANO, O ;
DEAN, J .
REPRODUCTION FERTILITY AND DEVELOPMENT, 1994, 6 (03) :319-330
1234567