Eugenol exhibits anti-virulence properties by competitively binding to quorum sensing receptors

被引：73

作者：

Rathinam, Prasanth ^{[1
]}

Kumar, H. S. Vijay ^{[2
]}

Viswanathan, Pragasam ^{[1
]}

机构：

[1] VIT Univ, Sch Biosci & Technol, Renal Res Lab, Ctr Biomed Res, Vellore, Tamil Nadu, India

[2] Maharani Lakshmi Ammanni Coll Women, Dept Biotechnol, Bangalore, Karnataka, India

来源：

BIOFOULING | 2017年 / 33卷 / 08期

关键词：

Catheter associated; urinary tract infections; Pseudomonas aeruginosa; quorum sensing; virulence; factors eugenol; biofilm; PSEUDOMONAS-AERUGINOSA; BIOLOGICAL EVALUATION; URINARY CATHETERS; BIOFILM FORMATION; SIGNAL MOLECULES; EXPRESSION; MODULATORS; INHIBITORS; INFECTION; EFFICACY;

D O I：

10.1080/08927014.2017.1350655

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The primary objective of this study was to ascertain the anti-biofilm and anti-virulence properties of sub-minimum inhibitory concentration (MIC) levels of eugenol against the standard strain PAO1 and two multi-drug resistant P. aeruginosa clinical isolates utilizing quorum sensing inhibition (QSI). Eugenol at 400 mu M significantly reduced biofilm formation on urinary catheters and the virulence factors (VF) including extracellular polysaccharides, rhamnolipid, elastase, protease, pyocyanin, and pyoverdine (p < 0.001). Further, eugenol exhibited a marked effect on the production of QS signals (AIs) (p < 0.001) without affecting their chemical integrity. In silico docking studies demonstrated a stable molecular binding between eugenol and QS receptor(s) in comparison with respective AIs. Investigation on reporter strains confirmed the competitive binding of eugenol to a QS receptor (LasR) as the possible QSI mechanism leading to significant repression of QS associated genes besides the VF genes (p < 0.001). This study provides insights, for the first time, into the mechanism of the anti-virulence properties of eugenol.

引用

页码：624 / 639

页数：16

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