Alkaloids of Phaedranassa dubia (Kunth) JF Macbr. and Phaedranassa brevifolia Meerow (Amaryllidaceae) from Ecuador and its cholinesterase-inhibitory activity

被引:10
作者
Acosta Leon, Karen [1 ]
Inca, Alexandra [1 ]
Tallini, Luciana R. [2 ,3 ]
Osorio, Edison H. [4 ]
Robles, Jessica [1 ]
Bastida, Jaume [2 ]
Oleas, Nora H. [5 ,6 ]
机构
[1] Escuela Super Politecn Chimborazo, Grp Invest Prod Nat & Farm, Fac Ciencias, Panamer Sur Km 1 1-2, EC-060155 Riobamba, Ecuador
[2] Univ Barcelona, Fac Farm & Ciencies Alimentacio, Dept Biol Sanitat & Medi Ambient, Grp Prod Nat, Av Joan XXIII 27-31, Barcelona 08028, Spain
[3] Univ Fed Rio Grande do Sul, Programa Posgrad Ciencias Farmaceut, Fac Farm, Av Ipiranga 2752, BR-90610000 Porto Alegre, RS, Brazil
[4] Univ Ibague, Fac Ciencias Nat & Matemat, Carrera 22 Calle 67, Ibague 730001, Colombia
[5] Univ Tecnol Indoamer, Fac Ciencias Medio Ambiente, Ctr Invest Biodiversidad & Cambia Climat BioCamb, EC-170301 Quito, Ecuador
[6] Univ Tecnol Indoamer, Fac Ciencias Medio Ambiente, Ingn Biodiversidad & Recursos Genet, EC-170301 Quito, Ecuador
关键词
AChE; Alkaloids; Alzheimer's disease; Amaryllidaceae; BuChE; Molecular docking; Phaedranassa; IDENTIFICATION; BUTYRYLCHOLINESTERASE; NARCISSUS; MONTANINE; REPLICATION; GALANTAMINE; APOPTOSIS; LYCORINE; AGENTS;
D O I
10.1016/j.sajb.2020.09.007
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Alzheimer's disease is considered the most common cause of dementia and, in an increasingly aging population worldwide, the quest for treatment is a priority. Amaryllidaceae alkaloids are of main interest because of their cholinesterase inhibition potential, which is the main palliative treatment available for this disease. We evaluated the alkaloidal profile and the in vitro inhibitory activity on acetylcholinesterase (AChE) and butyrylcholinesterase (BuChE) of bulb alkaloid extract of Phaedranassa dubia and Phaedranassa brevifolia collected in Ecuador. Using gas chromatography coupled to mass spectrometry (GC-MS), we identified typical Amaryllidaceae alkaloids in these species, highlighting the presence of lycorine-type alkaloids in P. dubia and haemanthamine/crinine-type in P. brevifolia. The species P. dubia and P. brevifolia showed inhibitory activities against AChE (IC50 values of 25.48 +/- 0.39 and 3.45 +/- 0.29 mu g.mL(-1), respectively) and BuChE (IC50 values of 114.96 +/- 4.94 and 58.89 +/- 0.55 mu g.mL(-1), respectively). Computational experiments allowed us to understand the interactions of the alkaloids identified in these samples toward the active sites of AChE and BuChE. In silico, some alkaloids detected in these Amaryllidaceae species presented higher estimated binding free energy toward BuChE than galanthamine. This is the first study about the alkaloid profile and biological potential of P. brevifolia species. (C) 2020 SAAB. Published by Elsevier B.V. All rights reserved.
引用
收藏
页码:91 / 99
页数:9
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