DNA-Directed Assembly of Carbon Nanotube-Protein Hybrids

被引：10
|
作者：
Freeley, Mark [1 ]
Gwyther, Rebecca E. A. [2 ]
Jones, D. Dafydd [2 ]
Palma, Matteo [1 ]
机构：
[1] Queen Mary Univ London, Dept Chem, London E1 4NS, England
[2] Cardiff Univ, Sch Biosci, Mol Biosci Div, Cardiff CF10 3AX, S Glam, Wales
来源：
BIOMOLECULES | 2021年 / 11卷 / 07期
基金：
英国生物技术与生命科学研究理事会;
关键词：
single-walled carbon nanotube; protein; DNA; hybrid; SWCNT; GFP; SINGLE-MOLECULE DETECTION; CHEMISTRY;
D O I：
10.3390/biom11070955
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Here, we report the controlled assembly of SWCNT-GFP hybrids employing DNA as a linker. Two distinct, enriched SWCNTs chiralities, (6,5), (7,6), and an unsorted SWCNT solution, were selectively functionalized with DNA and hybridized to a complementary GFP(DNA) conjugate. Atomic force microscopy images confirmed that GFP attachment occurred predominantly at the terminal ends of the nanotubes, as designed. The electronic coupling of the proteins to the nanotubes was confirmed via in-solution fluorescence spectroscopy, that revealed an increase in the emission intensity of GFP when linked to the CNTs.
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页数：9
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