New expression system to increase the yield of phloroglucinol

被引：3

作者：

Yuan Shan ^{[1
,2
]}

Zhang Rubing ^{[1
]}

Cao Yujin ^{[1
]}

Guo Jing ^{[1
]}

Xian Mo ^{[1
]}

Liu Wei ^{[1
]}

机构：

[1] Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, CAS Key Lab Biobased Mat, Qingdao 266101, Shandong, Peoples R China

[2] Univ Chinese Acad Sci, Beijing, Peoples R China

来源：

BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT | 2020年 / 34卷 / 01期

关键词：

Phloroglucinol; p-isopropylbenzoate; phlD1; rop; DIRECTED EVOLUTION; GENE-EXPRESSION; PCR;

D O I：

10.1080/13102818.2020.1764386

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Phloroglucinol (PG) is an important chemical that has been produced in engineered E. coli. Low yield and high cost are the bottlenecks that limit the industrialisation and popularisation of PG biosynthesis. In this study, a new expression system (pNEW vector) was developed for PG biosynthesis by using inexpensive p-isopropylbenzoate (cumate) as the inducer, and the copy number control element (rop) was added to optimise the original pNEW vector, which not only promoted the PG yield, but also reduced the leakage expression. Compared to traditional IPTG inducible expression system, 2-fold enhancement of PG yield was obtained with optimised expression system and the cost of inducer was reduced to 1/9. These results provide technical support for the biosynthesis of PG and reference for the selection of induced expression systems for other chemical biosynthesis.

引用

页码：405 / 412

页数：8

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