A Caged, Localizable Rhodamine Derivative for Superresolution Microscopy

被引：66

作者：

Banala, Sambashiva ^{[1
]}

Maurel, Damien ^{[1
]}

Manley, Suliana ^{[2
]}

Johnsson, Kai ^{[1
]}

机构：

[1] Ecole Polytech Fed Lausanne, Inst Chem Sci & Engn, Lausanne, Switzerland

[2] Ecole Polytech Fed Lausanne, Lab Expt Biophys, Lausanne, Switzerland

来源：

ACS CHEMICAL BIOLOGY | 2012年 / 7卷 / 02期

基金：

欧洲研究理事会; 瑞士国家科学基金会;

关键词：

FUSION PROTEINS; FLUOROPHORES; PROBES;

D O I：

10.1021/cb2002889

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A caged rhodamine 110 derivative for the specific labeling of SNAP-tag fusion proteins is introduced. The caged rhodamine 110 derivative permits the labeling of cell surface proteins in living cells and of intracellular proteins in fixed cells. The probe requires only a single caging group to maintain the fluorophore in a non-fluorescent state and becomes highly fluorescent after uncaging. The high contrast ratio is confirmed both in bulk and at the single molecule level. This property, together with its high photon yield makes it an excellent dye for photoactivated localization microscopy (PALM), as we demonstrate here.

引用

页码：288 / 292

页数：5

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