Effect of Chemotherapy on Whole-Blood Cytokine Responses to Mycobacterium tuberculosis Antigens in a Small Cohort of Patients with Pulmonary Tuberculosis

被引:14
作者
Bertholet, Sylvie [1 ]
Horne, David J. [2 ]
Laughlin, Elsa M. [1 ]
Savlov, Margery [2 ]
Tucakovic, Ines [1 ]
Coler, Rhea N. [1 ]
Narita, Masahiro [2 ,3 ]
Reed, Steven G. [1 ]
机构
[1] Infect Dis Res Inst, Seattle, WA 98104 USA
[2] Univ Washington, Sch Med, Dept Med, Div Pulm & Crit Care Med, Seattle, WA 98104 USA
[3] TB Control Program, Seattle, WA 98104 USA
基金
美国国家卫生研究院;
关键词
T-CELL RESPONSES; INTERFERON-GAMMA RESPONSES; IFN-GAMMA; ANTITUBERCULOSIS TREATMENT; ACTIVE TUBERCULOSIS; INFECTED INDIVIDUALS; IMMUNE-RESPONSE; RELEASE ASSAYS; DISEASE; ESAT-6;
D O I
10.1128/CVI.05037-11
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
The development of genomic and proteomic tools has enabled studies that begin to characterize the molecular targets of an effective host immune response to Mycobacterium tuberculosis, including understanding the specific immune responses associated with tuberculosis (TB) disease progression, disease resolution, and the development of latency. One application of such tools is the development of diagnostic reagents and assays useful as a test of cure. Such a test could be of considerable importance for the evaluation of new therapeutics. We and others have previously described immunodominant proteins of M. tuberculosis, including both vaccine and diagnostic candidates. In the present study, we describe the changes in immune responses to a panel of 71 M. tuberculosis antigens in six patients during the course of therapy. The levels of six cytokines were measured in 24-h whole-blood assays with these antigens, revealing that gamma interferon (IFN-gamma), tumor necrosis factor (TNF), and interleukin-10 (IL-10) were differentially regulated in response to a subset of antigens. Therefore, measuring the production of these three cytokines in response to a panel of carefully selected M. tuberculosis proteins during the course of TB therapy might be a promising path toward the development of a test of cure and warrants further validation in larger cohorts of pulmonary TB patients.
引用
收藏
页码:1378 / 1386
页数:9
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