mRNA with Mammalian Codon Bias Accumulates in Yeast Mutants with Constitutive Stress Granules

被引:4
作者
Kozlova, Natalia, V [1 ]
Pichon, Chantal [1 ,2 ]
Rahmouni, A. Rachid [1 ]
机构
[1] CNRS, Ctr Biophys Mol, UPR 4301, Rue Charles Sadron, F-45071 Orleans, France
[2] Univ Orleans, Colleguim Sci & Tech, F-45071 Orleans, France
关键词
stress granules; Saccharomyces cerevisiae; mRNA accumulation; SACCHAROMYCES-CEREVISIAE; GENE-EXPRESSION; PROTEIN; TRANSLATION; CELLS; BODY; DEADENYLATION; TRANSCRIPTION; DEGRADATION; STARVATION;
D O I
10.3390/ijms21041234
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Stress granules and P bodies are cytoplasmic structures assembled in response to various stress factors and represent sites of temporary storage or decay of mRNAs. Depending on the source of stress, the formation of these structures may be driven by distinct mechanisms, but several stresses have been shown to stabilize mRNAs via inhibition of deadenylation. A recent study identified yeast gene deletion mutants with constitutive stress granules and elevated P bodies; however, the mechanisms which trigger its formation remain poorly understood. Here, we investigate the possibility of accumulating mRNA with mammalian codon bias, which we termed the model RNA, in these mutants. We found that the model RNA accumulates in dcp2 and xrn1 mutants and in four mutants with constitutive stress granules overlapping with P bodies. However, in eight other mutants with constitutive stress granules, the model RNA is downregulated, or its steady state levels vary. We further suggest that the accumulation of the model RNA is linked to its protection from the main mRNA surveillance path. However, there is no obvious targeting of the model RNA to stress granules or P bodies. Thus, accumulation of the model RNA and formation of constitutive stress granules occur independently and only some paths inducing formation of constitutive stress granules will stabilize mRNA as well.
引用
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页数:20
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