mRNA with Mammalian Codon Bias Accumulates in Yeast Mutants with Constitutive Stress Granules
被引:4
作者:
Kozlova, Natalia, V
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机构:
CNRS, Ctr Biophys Mol, UPR 4301, Rue Charles Sadron, F-45071 Orleans, FranceCNRS, Ctr Biophys Mol, UPR 4301, Rue Charles Sadron, F-45071 Orleans, France
Kozlova, Natalia, V
[1
]
Pichon, Chantal
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机构:
CNRS, Ctr Biophys Mol, UPR 4301, Rue Charles Sadron, F-45071 Orleans, France
Univ Orleans, Colleguim Sci & Tech, F-45071 Orleans, FranceCNRS, Ctr Biophys Mol, UPR 4301, Rue Charles Sadron, F-45071 Orleans, France
Pichon, Chantal
[1
,2
]
Rahmouni, A. Rachid
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机构:
CNRS, Ctr Biophys Mol, UPR 4301, Rue Charles Sadron, F-45071 Orleans, FranceCNRS, Ctr Biophys Mol, UPR 4301, Rue Charles Sadron, F-45071 Orleans, France
Rahmouni, A. Rachid
[1
]
机构:
[1] CNRS, Ctr Biophys Mol, UPR 4301, Rue Charles Sadron, F-45071 Orleans, France
[2] Univ Orleans, Colleguim Sci & Tech, F-45071 Orleans, France
Stress granules and P bodies are cytoplasmic structures assembled in response to various stress factors and represent sites of temporary storage or decay of mRNAs. Depending on the source of stress, the formation of these structures may be driven by distinct mechanisms, but several stresses have been shown to stabilize mRNAs via inhibition of deadenylation. A recent study identified yeast gene deletion mutants with constitutive stress granules and elevated P bodies; however, the mechanisms which trigger its formation remain poorly understood. Here, we investigate the possibility of accumulating mRNA with mammalian codon bias, which we termed the model RNA, in these mutants. We found that the model RNA accumulates in dcp2 and xrn1 mutants and in four mutants with constitutive stress granules overlapping with P bodies. However, in eight other mutants with constitutive stress granules, the model RNA is downregulated, or its steady state levels vary. We further suggest that the accumulation of the model RNA is linked to its protection from the main mRNA surveillance path. However, there is no obvious targeting of the model RNA to stress granules or P bodies. Thus, accumulation of the model RNA and formation of constitutive stress granules occur independently and only some paths inducing formation of constitutive stress granules will stabilize mRNA as well.
机构:
Univ Colorado, Dept Chem & Biochem, Boulder, CO 80303 USA
Univ Colorado, Howard Hughes Med Inst, Boulder, CO 80303 USAUniv Colorado, Dept Chem & Biochem, Boulder, CO 80303 USA
Buchan, J. Ross
;
Kolaitis, Regina-Maria
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机构:
St Jude Childrens Res Hosp, Dept Dev Neurobiol, Memphis, TN 38105 USAUniv Colorado, Dept Chem & Biochem, Boulder, CO 80303 USA
Kolaitis, Regina-Maria
;
Taylor, J. Paul
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机构:
St Jude Childrens Res Hosp, Dept Dev Neurobiol, Memphis, TN 38105 USAUniv Colorado, Dept Chem & Biochem, Boulder, CO 80303 USA
Taylor, J. Paul
;
Parker, Roy
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机构:
Univ Colorado, Dept Chem & Biochem, Boulder, CO 80303 USA
Univ Colorado, Howard Hughes Med Inst, Boulder, CO 80303 USAUniv Colorado, Dept Chem & Biochem, Boulder, CO 80303 USA
机构:
Univ Arizona, Howard Hughes Med Inst, Tucson, AZ 85721 USA
Univ Arizona, Dept Mol & Cellular Biol, Tucson, AZ 85721 USAUniv Arizona, Howard Hughes Med Inst, Tucson, AZ 85721 USA
Buchan, J. Ross
;
Yoon, Je-Hyun
论文数: 0引用数: 0
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机构:
Univ Arizona, Dept Mol & Cellular Biol, Tucson, AZ 85721 USAUniv Arizona, Howard Hughes Med Inst, Tucson, AZ 85721 USA
Yoon, Je-Hyun
;
Parker, Roy
论文数: 0引用数: 0
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机构:
Univ Arizona, Howard Hughes Med Inst, Tucson, AZ 85721 USA
Univ Arizona, Dept Mol & Cellular Biol, Tucson, AZ 85721 USAUniv Arizona, Howard Hughes Med Inst, Tucson, AZ 85721 USA
机构:
Univ Arizona, Howard Hughes Med Inst, Tucson, AZ 85721 USA
Univ Arizona, Dept Mol & Cell Biol, Tucson, AZ 85721 USAUniv Arizona, Howard Hughes Med Inst, Tucson, AZ 85721 USA
Buchan, J. Ross
;
Parker, Roy
论文数: 0引用数: 0
h-index: 0
机构:
Univ Arizona, Howard Hughes Med Inst, Tucson, AZ 85721 USA
Univ Arizona, Dept Mol & Cell Biol, Tucson, AZ 85721 USAUniv Arizona, Howard Hughes Med Inst, Tucson, AZ 85721 USA
机构:
Univ Colorado, Dept Chem & Biochem, Boulder, CO 80303 USA
Univ Colorado, Howard Hughes Med Inst, Boulder, CO 80303 USAUniv Colorado, Dept Chem & Biochem, Boulder, CO 80303 USA
Buchan, J. Ross
;
Kolaitis, Regina-Maria
论文数: 0引用数: 0
h-index: 0
机构:
St Jude Childrens Res Hosp, Dept Dev Neurobiol, Memphis, TN 38105 USAUniv Colorado, Dept Chem & Biochem, Boulder, CO 80303 USA
Kolaitis, Regina-Maria
;
Taylor, J. Paul
论文数: 0引用数: 0
h-index: 0
机构:
St Jude Childrens Res Hosp, Dept Dev Neurobiol, Memphis, TN 38105 USAUniv Colorado, Dept Chem & Biochem, Boulder, CO 80303 USA
Taylor, J. Paul
;
Parker, Roy
论文数: 0引用数: 0
h-index: 0
机构:
Univ Colorado, Dept Chem & Biochem, Boulder, CO 80303 USA
Univ Colorado, Howard Hughes Med Inst, Boulder, CO 80303 USAUniv Colorado, Dept Chem & Biochem, Boulder, CO 80303 USA
机构:
Univ Arizona, Howard Hughes Med Inst, Tucson, AZ 85721 USA
Univ Arizona, Dept Mol & Cellular Biol, Tucson, AZ 85721 USAUniv Arizona, Howard Hughes Med Inst, Tucson, AZ 85721 USA
Buchan, J. Ross
;
Yoon, Je-Hyun
论文数: 0引用数: 0
h-index: 0
机构:
Univ Arizona, Dept Mol & Cellular Biol, Tucson, AZ 85721 USAUniv Arizona, Howard Hughes Med Inst, Tucson, AZ 85721 USA
Yoon, Je-Hyun
;
Parker, Roy
论文数: 0引用数: 0
h-index: 0
机构:
Univ Arizona, Howard Hughes Med Inst, Tucson, AZ 85721 USA
Univ Arizona, Dept Mol & Cellular Biol, Tucson, AZ 85721 USAUniv Arizona, Howard Hughes Med Inst, Tucson, AZ 85721 USA
机构:
Univ Arizona, Howard Hughes Med Inst, Tucson, AZ 85721 USA
Univ Arizona, Dept Mol & Cell Biol, Tucson, AZ 85721 USAUniv Arizona, Howard Hughes Med Inst, Tucson, AZ 85721 USA
Buchan, J. Ross
;
Parker, Roy
论文数: 0引用数: 0
h-index: 0
机构:
Univ Arizona, Howard Hughes Med Inst, Tucson, AZ 85721 USA
Univ Arizona, Dept Mol & Cell Biol, Tucson, AZ 85721 USAUniv Arizona, Howard Hughes Med Inst, Tucson, AZ 85721 USA